PRKAA2 Antibody (OAAB06774)

Catalog No: OAAB06774

Size:400ul

PRKAA2 antibody (Cat. #OAAB06774) western blot analysis in CEM cell line lysates (35ug/lane).This demonstrates the PRKAA2 antibody detected the PRKAA2 protein (arrow).

Product Info

• Predicted Species Reactivity: Human
• Product Format: Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, followed by dialysis against PBS.
• Clonality: Monoclonal
• Clone: 206CT5.4.2
• Isotype: IgG2a,K
• Host: Mouse
• Application: WB
• Additional Information: Precautions: PRKAA2 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
  Function: Catalytic subunit of AMP-activated protein kinase (AMPK), an energy sensor protein kinase that plays a key role in regulating cellular energy metabolism. In response to reduction of intracellular ATP levels, AMPK activates energy-producing pathways and inhibits energy-consuming processes: inhibits protein, carbohydrate and lipid biosynthesis, as well as cell growth and proliferation. AMPK acts via direct phosphorylation of metabolic enzymes, and by longer-term effects via phosphorylation of transcription regulators. Also acts as a regulator of cellular polarity by remodeling the actin cytoskeleton; probably by indirectly activating myosin. Regulates lipid synthesis by phosphorylating and inactivating lipid metabolic enzymes such as ACACA, ACACB, GYS1, HMGCR and LIPE; regulates fatty acid and cholesterol synthesis by phosphorylating acetyl-CoA carboxylase (ACACA and ACACB) and hormone-sensitive lipase (LIPE) enzymes, respectively. Regulates insulin-signaling and glycolysis by phosphorylating IRS1, PFKFB2 and PFKFB3. AMPK stimulates glucose uptake in muscle by increasing the translocation of the glucose transporter SLC2A4/GLUT4 to the plasma membrane, possibly by mediating phosphorylation of TBC1D4/AS160. Regulates transcription and chromatin structure by phosphorylating transcription regulators involved in energy metabolism such as CRTC2/TORC2, FOXO3, histone H2B, HDAC5, MEF2C, MLXIPL/ChREBP, EP300, HNF4A, p53/TP53, SREBF1, SREBF2 and PPARGC1A. Acts as a key regulator of glucose homeostasis in liver by phosphorylating CRTC2/TORC2, leading to CRTC2/TORC2 sequestration in the cytoplasm. In response to stress, phosphorylates 'Ser-36' of histone H2B (H2BS36ph), leading to promote transcription. Acts as a key regulator of cell growth and proliferation by phosphorylating TSC2, RPTOR and ATG1: in response to nutrient limitation, negatively regulates the mTORC1 complex by phosphorylating RPTOR component of the mTORC1 complex and by phosphorylating and activating TSC2. In response to nutrient limitation, promotes autophagy by phosphorylating and activating ULK1. AMPK also acts as a regulator of circadian rhythm by mediating phosphorylation of CRY1, leading to destabilize it May regulate the Wnt signaling pathway by phosphorylating CTNNB1, leading to stabilize it. Also phosphorylates CFTR, EEF2K, KLC1, NOS3 and SLC12A1
  Cellular Location: Cytoplasm (By similarity). Nucleus. Note=In response to stress, recruited by p53/TP53 to specific promoters
• Reconstitution and Storage: Maintain refrigerated at 2-8C for up to 2 weeks. For long term storage store at -20C in small aliquots to prevent freeze-thaw cycles.
• Immunogen: This PRKAA2 monoclonal antibody is generated from mouse immunized with PRKAA2 recombinant protein.
• Predicted Homology Based on Immunogen Sequence: Human
• Concentration: Approximately 0.5mg/ml. Actual concentration varies with each lot.
• Application Info: WB: 1:1000
  IF: 1:10~50

Target Info

• Gene Symbol: PRKAA2
• Alias Symbols: AMPK, AMPK2, PRKAA, AMPKa2
• NCBI Gene Id: 5563
• Protein Name: 5'-AMP-activated protein kinase catalytic subunit alpha-2
• Description of Target: Mouse Monoclonal Antibody (Mab)
• Uniprot ID: P54646
• Protein Accession #: NP_006243.2
• Molecular Weight: 62320 Da