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Datasheets/Manuals | Printable datasheet for anti-PRKAA1 (OASG00373) antibody |
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Predicted Species Reactivity | Human, Mouse, Rat, Monkey |
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Product Format | Liquid. PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
Clonality | Polyclonal |
Isotype | IgG |
Host | Rabbit |
Application | WB, IHC |
Additional Information | Function: Catalytic subunit of AMP-activated protein kinase (AMPK), an energy sensor protein kinase that plays a key role in regulating cellular energy metabolism. In response to reduction of intracellular ATP levels, AMPK activates energy-producing pathways and inhibits energy-consuming processes: inhibits protein, carbohydrate and lipid biosynthesis, as well as cell growth and proliferation. AMPK acts via direct phosphorylation of metabolic enzymes, and by longer-term effects via phosphorylation of transcription regulators. Cellular Localization: Cytoplasm Nucleus. In response to stress, recruited by p53/TP53 to specific promoters. |
Reconstitution and Storage | Store at -20C. Avoid repeated freeze/thaw cycles. |
Immunogen | Synthesized peptide derived between 110-190aa from human AMPK Alpha1/2 around the phosphorylation site of T183/172. |
Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
Concentration | 1 mg/ml |
Target Post-Translational Modification | Ubiquitinated. Phosphorylated at Thr-183 by STK11/LKB1 in complex with STE20-related adapter-alpha (STRADA) pseudo kinase and CAB39. Also phosphorylated at Thr-183 by CAMKK2; triggered by a rise in intracellular calcium ions, without detectable changes in the AMP/ATP ratio. CAMKK1 can also phosphorylate Thr-183, but at a much lower level. Dephosphorylated by protein phosphatase 2A and 2C (PP2A and PP2C). Phosphorylated by ULK1 and ULK2; leading to negatively regulate AMPK activity and suggesting the existence of a regulatory feedback loop between ULK1, ULK2 and AMPK. Dephosphorylated by PPM1A and PPM1B. |
Specificity | Phospho-AMPK Alpha1/2 (T183/172) Polyclonal Antibody detects endogenous levels of AMPK Alpha1/2 protein only when phosphorylated at T183/172. |
Application Info | WB: 1:500-1:2000 IHC: 1:100-1:300 ELISA: 1:40000 |
Gene Symbol | PRKAA1, PRKAA2 |
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Gene Full Name | protein kinase, AMP-activated, alpha 1 catalytic subunit |
Alias Symbols | AMPK, AMPKa1, AMPK alpha 1 |
NCBI Gene Id | 5562; 5563; 105787; 108079; 65248; 78975 |
Description of Target | The protein encoded by this gene belongs to the ser/thr protein kinase family. It is the catalytic subunit of the 5'-prime-AMP-activated protein kinase (AMPK). AMPK is a cellular energy sensor conserved in all eukaryotic cells. The kinase activity of AMPK is activated by the stimuli that increase the cellular AMP/ATP ratio. AMPK regulates the activities of a number of key metabolic enzymes through phosphorylation. It protects cells from stresses that cause ATP depletion by switching off ATP-consuming biosynthetic pathways. Alternatively spliced transcript variants encoding distinct isoforms have been observed. |
Uniprot ID | Q13131, P54646, Q5EG47, Q8BRK8, P54645, Q09137 |
Molecular Weight | 63 kDa |
- Protocol:
- Reconstitution & Storage Instructions
- Western Blotting/Immunoblotting (WB/IB) Protocol
- Immunohistochemistry (IHC) Protocol
- Immunocytochemistry (ICC) Protocol
- Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
- Blocking Peptide Competition Protocol (BPCP)
- Immunoprecipitation (IP) Protocol
- Antibody Array (AA) Protocol
- Tips Information:
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See our General FAQ page.
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What is the species homology for "PRKAA1 Antibody (Phospho-Thr183/172) (OASG00373)"?
The tested species reactivity for this item is "". This antibody is predicted to have homology to "Human, Mouse, Rat, Monkey".
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How long will it take to receive "PRKAA1 Antibody (Phospho-Thr183/172) (OASG00373)"?
This item is available "Domestic: within 2-3 weeks delivery | International: 2-3 weeks".
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What buffer format is "PRKAA1 Antibody (Phospho-Thr183/172) (OASG00373)" provided in?
This item is provided in "Liquid. PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.".
Additional format options may be available. For more information please contact info@avivasysbio.com. -
What are other names for "PRKAA1 Antibody (Phospho-Thr183/172) (OASG00373)"?
This target may also be called "AMPK, AMPKa1, AMPK alpha 1" in publications.
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What is the shipping cost for "PRKAA1 Antibody (Phospho-Thr183/172) (OASG00373)"?
The shipping cost for this item is $40 within the US. Please contact us for specific shipping prices for international orders.
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What is the guarantee for "PRKAA1 Antibody (Phospho-Thr183/172) (OASG00373)"?
All Aviva products have been through rigorous validations and carry 100% satisfaction guarantee.
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Can I get bulk pricing for "PRKAA1 Antibody (Phospho-Thr183/172) (OASG00373)"?
You can get bulk pricing for this item by going here.
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What is the molecular weight of the protein?
The molecular weight reported by Uniprot for this item is "63 kDa".
Please note observed molecular weights in western blot applications may differ depending on a variety of protein characteristics. -
What protocols are available for "PRKAA1 Antibody (Phospho-Thr183/172) (OASG00373)"?
We may have detailed protocol data avaialble for this item. To learn more, please view the "Protocols & Data" tab on the product page.
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What are positive controls for "PRKAA1, PRKAA2"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
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What are negative controls for "PRKAA1, PRKAA2"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
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What other proteins interact with "PRKAA1, PRKAA2"?
This protein has been reported to interact with "Protein Interactions". Please view the "Related Categories" tab on the product page for more information.
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What biological processes are associated with "PRKAA1, PRKAA2"?
This protein has been associated with "Biological Processes". Please view the "Related Categories" tab on the product page for more information.
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What cellular components are associated with "PRKAA1, PRKAA2"?
This protein has been associated with "Cellular Components". Please view the "Related Categories" tab on the product page for more information.
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What protein functions are associated with "PRKAA1, PRKAA2"?
This protein has been associated with "Protein Functions". Please view the "Related Categories" tab on the product page for more information.