PRKAA1 Antibody (OACA09894)

Catalog No: OACA09894

Immunofluorescent analysis of Hela cells at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)

Immunohistochemistry of paraffin-embedded human lung tissue at dilution of 1:100

Immunohistochemistry of paraffin-embedded human ovarian cancer at dilution of 1:100

Western Blot
Positive WB detected in: Mouse spleen tissue,MCF-7 whole cell lysate
All lanes: AMPK1 antibody at 3ug/ml
Secondary
Goat polyclonal to rabbit IgG at 1/5,0000 dilution
Predicted band size: 65,66 kDa
Observed band size: 65 kDa

Western Blot
Positive WB detected in: Mouse spleen tissue,MCF-7 whole cell lysate
All lanes: AMPK1 antibody at 3ug/ml
Secondary
Goat polyclonal to rabbit IgG at 1/5,0000 dilution
Predicted band size: 65,66 kDa
Observed band size: 65 kDa

Immunohistochemistry of paraffin-embedded human lung tissue at dilution of 1:100

Immunohistochemistry of paraffin-embedded human ovarian cancer at dilution of 1:100

Immunofluorescent analysis of Hela cells at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)

Product Info

• Predicted Species Reactivity: Human|Mouse
• Product Format: Liquid
• Clonality: Polyclonal
• Isotype: IgG
• Host: Rabbit
• Conjugation: Unconjugated
• Application: Enzyme-linked immunosorbent assay|Immunofluorescence|Immunohistochemistry|Western blot
• Reconstitution and Storage: -20°C or -80°C
• Immunogen: Recombinant Human 5\\\'-AMP-activated protein kinase catalytic subunit alpha-1 protein (325-543AA)
• Purification: Protein G Purified
• Preservative: 0.03% Proclin 300
• Formulation: 50% Glycerol, 0.01M PBS, PH 7.4
• Purity: >95%

Target Info

• Gene Symbol: PRKAA1
• Gene Full Name: protein kinase AMP-activated catalytic subunit alpha 1
• Alias Symbols: 5'-AMP-activated protein kinase catalytic subunit alpha-1;5'-AMP-activated protein kinase, catalytic alpha-1 chain;ACACA kinase;acetyl-CoA carboxylase kinase;AMP -activate kinase alpha 1 subunit;AMP-activated protein kinase, catalytic, alpha-1;AMPK;AMPK alpha 1;AMPK subunit alpha-1;AMPK, alpha, 1;AMPKa1;HMGCR kinase;hydroxymethylglutaryl-CoA reductase kinase;protein kinase, AMP-activated, alpha 1 catalytic subunit;tau-protein kinase PRKAA1.
• NCBI Gene Id: 5562
• Protein Name: 5'-AMP-activated protein kinase catalytic subunit alpha-1
• Description of Target: Catalytic subunit of AMP-activated protein kinase (AMPK), an energy sensor protein kinase that plays a key role in regulating cellular energy metabolism. In response to reduction of intracellular ATP levels, AMPK activates energy-producing pathways and inhibits energy-consuming processes: inhibits protein, carbohydrate and lipid biosynthesis, as well as cell growth and proliferation. AMPK acts via direct phosphorylation of metabolic enzymes, and by longer-term effects via phosphorylation of transcription regulators. Also acts as a regulator of cellular polarity by remodeling the actin cytoskeleton; probably by indirectly activating myosin. Regulates lipid synthesis by phosphorylating and inactivating lipid metabolic enzymes such as ACACA, ACACB, GYS1, HMGCR and LIPE; regulates fatty acid and cholesterol synthesis by phosphorylating acetyl-CoA carboxylase (ACACA and ACACB) and hormone-sensitive lipase (LIPE) enzymes, respectively. Regulates insulin-signaling and glycolysis by phosphorylating IRS1, PFKFB2 and PFKFB3. AMPK stimulates glucose uptake in muscle by increasing the translocation of the glucose transporter SLC2A4/GLUT4 to the plasma membrane, possibly by mediating phosphorylation of TBC1D4/AS160. Regulates transcription and chromatin structure by phosphorylating transcription regulators involved in energy metabolism such as CRTC2/TORC2, FOXO3, histone H2B, HDAC5, MEF2C, MLXIPL/ChREBP, EP300, HNF4A, p53/TP53, SREBF1, SREBF2 and PPARGC1A. Acts as a key regulator of glucose homeostasis in liver by phosphorylating CRTC2/TORC2, leading to CRTC2/TORC2 sequestration in the cytoplasm. In response to stress, phosphorylates 'Ser-36' of histone H2B (H2BS36ph), leading to promote transcription. Acts as a key regulator of cell growth and proliferation by phosphorylating TSC2, RPTOR and ATG1/ULK1: in response to nutrient limitation, negatively regulates the mTORC1 complex by phosphorylating RPTOR component of the mTORC1 complex and by phosphorylating and activating TSC2. In response to nutrient limitation, promotes autophagy by phosphorylating and activating ATG1/ULK1. In response to nutrient limitation, phosphorylates transcription factor FOXO3 promoting FOXO3 mitochontrial import (By similarity). AMPK also acts as a regulator of circadian rhythm by mediating phosphorylation of CRY1, leading to destabilize it. May regulate the Wnt signaling pathway by phosphorylating CTNNB1, leading to stabilize it. Also has tau-protein kinase activity: in response to amyloid beta A4 protein (APP) exposure, activated by CAMKK2, leading to phosphorylation of MAPT/TAU; however the relevance of such data remains unclear in vivo. Also phosphorylates CFTR, EEF2K, KLC1, NOS3 and SLC12A1.
• Uniprot ID: Q13131
• Protein Accession #: NP_006242
• Nucleotide Accession #: NM_006251