PRG4 ELISA Kit (Mouse) (OKCD08903)

Catalog No: OKCD08903

Size:96WELLS

PRG4 ELISA Kit (Mouse) (OKCD08903) Standard Curve

Product Info

• Predicted Species Reactivity: Mouse|Mus musculus
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Proteoglycan 4 (PRG4). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Proteoglycan 4 (PRG4). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Proteoglycan 4 (PRG4), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Proteoglycan 4 (PRG4) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 0.156-10ng/mL
• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Proteoglycan 4 (PRG4) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Proteoglycan 4 (PRG4) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • PRG4 Microplate: 96 Wells (12 x 8 Well strips)
  • PRG4 Lyophilized Standard: 2
  • 100X PRG4 HRP-Detector Anitbody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 9 mL
  • Stop Solution: 1 x 6 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Serum, plasma, synovial fluid and other biological fluids
• Sensitivity: < 0.065ng/mL

Target Info

• Gene Symbol: Prg4
• Gene Full Name: proteoglycan 4 (megakaryocyte stimulating factor, articular superficial zone protein)
• Alias Symbols: CACP;DOL;DOL54;JCAP;lub;lubricin;megakaryocyte-stimulating factor;MS;MSF;proteoglycan 3;proteoglycan 4;S;superficial zone proteoglycan;SZP.
• NCBI Gene Id: 96875
• Protein Name: Proteoglycan 4
• Description of Target: Plays a role in boundary lubrication within articulating joints. Prevents protein deposition onto cartilage from synovial fluid by controlling adhesion-dependent synovial growth and inhibiting the adhesion of synovial cells to the cartilage surface.
• Uniprot ID: Q9JM99
• Protein Accession #: NP_001103616.1
• Nucleotide Accession #: NM_001110146.1