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Catalog No: OKCD00840 (Formerly GWB-KBBEA9)
Size:96WELLS
Price: $650.00
SKU
OKCD00840
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Datasheets/ManualsPrintable datasheet for PRG2 ELISA Kit (Mouse) (OKCD00840)
Product Info
Predicted Species ReactivityMouse|Mus musculus
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric
ELISA Kit Duration3h
ELISA Kit Linearity
Sample1:2 Dilution1:4 Dilution1:8 Dilution1:16 Dilution
serum(n=5)86-94%97-104%79-104%80-101%
EDTA plasma(n=5)84-92%79-104%78-105%79-91%
heparin plasma(n=5)85-99%97-104%80-90%87-94%
ELISA Kit PrincipleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Major Basic Protein (MBP). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Major Basic Protein (MBP). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Major Basic Protein (MBP), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Major Basic Protein (MBP) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
ELISA Kit Range0.312-20ng/mL
ELISA Kit Recovery
MatrixRecovery Range (%)Average (%)
serum(n=5)83-10189
EDTA plasma(n=5)89-9693
heparin plasma(n=5)94-10198
ELISA Kit ReproducibilityIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Major Basic Protein (MBP) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Major Basic Protein (MBP) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
ELISA Kit Component
ComponentAmount
Anti-Prg2 Microplate96 Wells (12 x 8 Well Strips)
Prg2 Lyophilized Standard2 x 20 ng
100X Biotinylated Prg2 Detector Antibody120 uL
Avidin/HRP Conjugate120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Reconstitution and Storage2°C to 8°C|-20°C
Sample TypeSerum, plasma, tissue homogenates, cell lysates and other biological fluids
Sensitivity< 0.114 ng/mL
SpecificityThis assay has high sensitivity and excellent specificity for detection of Major Basic Protein (MBP). No significant cross-reactivity or interference between Major Basic Protein (MBP) and analogues was observed.
Assay InfoAssay Methodology: Quantitative Sandwich Immunoassay
Gene SymbolPrg2
Gene Full Nameproteoglycan 2, bone marrow
Alias SymbolsBMPG;bone marrow proteoglycan;EMB;EMBP;major basic protein 1;MBP;mMB;mMBP;mMBP-;mMBP-1;Proteoglycan 2.
NCBI Gene Id19074
Protein NameBone marrow proteoglycan
Description of TargetCytotoxin and helminthotoxin. MBP also induces non-cytolytic histamine release from basophils. It is involved in antiparasitic defense mechanisms and immune hypersensitivity reactions (By similarity).
Uniprot IDQ61878
Protein Accession #NP_032946
Nucleotide Accession #NM_008920.4
Protein Size (# AA)223
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