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Catalog No: OKCD03736
Size:96WELLS
Price: $850.00
SKU
OKCD03736
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PRG2 Chemi-Luminescent ELISA Kit (Mouse) (OKCD03736)

Datasheets/ManualsPrintable datasheet for PRG2 Chemi-Luminescent ELISA Kit (Mouse) (OKCD03736)
Product Info
Predicted Species ReactivityMouse|Mus musculus
ApplicationChemiluminescent immunoassay
ELISA Kit Detection MethodChemiluminescent
ELISA Kit Duration2h, 40min
ELISA Kit Linearity
Sample1:21:41:81:16
Serum (n=5)84-92%95-105%78-91%92-99%
EDTA Plasma (n=5)84-96%99-105%98-105%79-88%
Heparin Plasma (n=5)97-105%98-105%89-97%98-105%
ELISA Kit PrincipleThe microplate provided in this kit has been pre-coated with an antibody specific to Major Basic Protein (MBP). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Major Basic Protein (MBP). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Major Basic Protein (MBP) level in the sample or standard.
ELISA Kit Range54.9-40,000pg/mL
ELISA Kit Recovery
MatrixRecovery range (%)Average(%)
Serum (n=5)80-8984
EDTA Plasma (n=5)80-10195
Heparin Plasma (n=5)97-104101
ELISA Kit ReproducibilityIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Major Basic Protein (MBP) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Major Basic Protein (MBP) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
ELISA Kit Component
ComponentAmount
Prg2 Microplate96 Wells (12 x 8 Well strips)
Prg2 Lyophilized Standard2
100X Prg2 Biotinylated Detector Anitbody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
100X Luminol Substrate1 x 2 mL
Substrate Diluent1 x 20 mL
Reconstitution and Storage2°C to 8°C|-20°C
Sample TypeSerum, plasma and other biological fluids
Sensitivity0.625 ng/mL
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolPrg2
Gene Full Nameproteoglycan 2, bone marrow
Alias SymbolsBMPG;bone marrow proteoglycan;EMB;EMBP;major basic protein 1;MBP;mMB;mMBP;mMBP-;mMBP-1;Proteoglycan 2.
NCBI Gene Id19074
Protein NameBone marrow proteoglycan
Description of TargetCytotoxin and helminthotoxin. MBP also induces non-cytolytic histamine release from basophils. It is involved in antiparasitic defense mechanisms and immune hypersensitivity reactions (By similarity).
Uniprot IDQ61878
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