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Catalog No: OKEH00559
Size:96T
Price: $525.00
SKU
OKEH00559
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
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PRF1 ELISA Kit (Human) : 96 Wells (OKEH00559)

Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityHomo sapiens, Human
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~ 3 Hours
ELISA Kit PrincipleAviva Systems Biology PRF1 ELISA Kit (Human) (OKEH00559) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Human PRF1 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. After washing,a biotinylated detector antibody specific for Human PRF1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away.An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Human PRF1 captured in well.
ELISA Kit Range15.6-1000pg/mL
ELISA Kit RecoveryMean recovery when spiking into sample matrices at concentrations within the dynamic range: 95%
ELISA Kit ReproducibilityMean Intra-assay CV%: < 5.5% (n = 20)
Mean Inter-assay CV%: < 9.6% (n = 20)
ELISA Kit Component
ComponentAmount
PRF1 Microplate96 Wells (12 x 8 Well strips)
PRF1 Lyophilized Standard2
100X Biotinylated PRF1 Detector Antibody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Sample Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Tissue Homogenates, Cell Culture Supernates, Other Biological Fluids
Sensitivity10 pg/mL
SpecificityNatural and recombinant Human Perforin-1
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolPRF1
Gene Full Nameperforin 1
Alias Symbolscytolysin;HPLH2;lymphocyte pore-forming protein;P1;perforin 1 (pore forming protein);perforin-1;PFP.
NCBI Gene Id5551
Protein NameP14222
Description of TargetPerforin-1
Uniprot IDhttps://www.uniprot.org/uniprot/P14222
Protein Accession #NP_001076585.1
Nucleotide Accession #NM_001083116.1
Protein Size (# AA)555
Molecular Weight61 kDa
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