Catalog No: OKCD01952 (Formerly GWB-KBBPS7)
Size:96WELLS
Price: $675.00
SKU
OKCD01952
Availability: Domestic: within 1-2 weeks delivery | International: within 1-2 weeks delivery
Please contact customer service when ordering 10+ kits.

Bulk
Order
Aviva's
Satisfaction
Guarantee
Contact Us:
Shipping Info:
  • $55: Antibody & Protein in US
  • $55 + $25/Kit in US
  • Contact us for international orders.
Datasheets/ManualsPrintable datasheet for PRELP ELISA Kit (Human) (OKCD01952)
Product Info
Predicted Species ReactivityHomo sapiens|Human
ApplicationELISA-Sandwich
ELISA Kit Detection MethodColorimetric
ELISA Kit Duration3h
ELISA Kit Linearity
Sample1:2 Dilution1:4 Dilution1:8 Dilution1:16 Dilution
Serum (n=5)80-101%95-104%83-94%82-94%
EDTA Plasma (n=5)78-94%93-104%89-103%79-99%
Heparin Plasma (n=5)95-102%93-102%82-102%78-93%
ELISA Kit PrincipleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Proline Arginine Rich End Leucine Rich Repeat Protein (PRELP). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Proline Arginine Rich End Leucine Rich Repeat Protein (PRELP). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Proline Arginine Rich End Leucine Rich Repeat Protein (PRELP), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Proline Arginine Rich End Leucine Rich Repeat Protein (PRELP) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
ELISA Kit Range3.12-200ng/mL
ELISA Kit Recovery
MatrixRecovery Range (%)Average (%)
Serum (n=5)98-105101
EDTA Plasma (n=5)84-9489
Heparin Plasma (n=5)81-9387
ELISA Kit ReproducibilityIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Proline Arginine Rich End Leucine Rich Repeat Protein (PRELP) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Proline Arginine Rich End Leucine Rich Repeat Protein (PRELP) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
ELISA Kit Component
ComponentAmount
Anti-PRELP Microplate96 Wells (12 x 8 Well Strips)
PRELP Lyophilized Standard2 x 20 ng
100X Biotinylated PRELP Detector Antibody120 uL
Avidin/HRP Conjugate120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Reconstitution and Storage2°C to 8°C|-20°C
Sample TypeSerum, plasma, tissue homogenates and other biological fluids
Sensitivity< 0.129 ng/mL
SpecificityThis assay has high sensitivity and excellent specificity for detection of Proline Arginine Rich End Leucine Rich Repeat Protein (PRELP). No significant cross-reactivity or interference between Proline Arginine Rich End Leucine Rich Repeat Protein (PRELP) and analogues was observed.
Assay InfoAssay Methodology: Quantitative Sandwich Immunoassay
Gene SymbolPRELP
Gene Full Nameproline and arginine rich end leucine rich repeat protein
Alias Symbols55 kDa leucine-rich repeat protein of articular cartilage;MST161;MSTP161;prolargin;prolargin proteoglycan;proline-arginine-rich end leucine-rich repeat protein;SLRR2A.
NCBI Gene Id5549
Protein NameProlargin
Description of TargetMay anchor basement membranes to the underlying connective tissue.
Uniprot IDP51888
Protein Accession #NP_002716.1
Nucleotide Accession #NM_002725.3
Protein Size (# AA)382
Write Your Own Review
You're reviewing:PRELP ELISA Kit (Human) (OKCD01952)
Your Rating
We found other products you might like!