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Catalog No: OKIA00111 (Formerly GWB-374Z37)
Size:1x96WELLPLATE
Price: $509.00
SKU
OKIA00111
Availability: Domestic: within 1 week delivery | International: 1 week
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  • $55: Antibody & Protein in US
  • $55 + $25/Kit in US
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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Please click here to view the MSDS.
COA DatasheetPrintable COA datasheet for OKIA00111
Product Info
Predicted Species ReactivityMouse
Product FormatIn Solution
ApplicationELISA
ELISA Kit Detection MethodColorimetric, OD 450 nm
ELISA Kit Duration60 Minutes
ELISA Kit PrincipleAviva Systems Biology Prealbumin ELISA Kit (Mouse) (OKIA00111) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody for Transthyretin has been pre-coated onto a 96-wellplate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and washed to remove unbound proteins. An Anti-Transthyretin - HRP conjugated detector antibody is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Transthyretin captured in the well.
ELISA Kit Range15.6 ng/ml - 500 ng/ml
ELISA Kit Component
ComponentAmount
Anti-Mouse Prealbumin (Transthyretin) Microplate96 Wells (12 x 8 Well Strips)
5X Diluent Concentrate50 mL
20X Wash Solution50 mL
100X Enzyme-Antibody Conjugate150 uL
Chromogen-Substrate Solution12 mL
Stop Solution12 mL
Mouse Prealbumin (Transthyretin) Calibrator1
Additional InformationIntended Use: The Prealbumin test kits are a highly sensitive two-site enzyme linked immunoassay (ELISA) for measuring Prealbumin in biological samples of mice.
::Reagents:
1. DILUENT CONCENTRATE (Running Buffer)
One bottle containing 50 ml of a 5X concentrated diluent running buffer.
2. WASH SOLUTION CONCENTRATE
One bottle containing 50 ml of a 20X concentrated wash solution.
3. ENZYME-ANTIBODY CONJUGATE 100X
One vial containing 150 ul of affinity purified anti-Mouse Prealbumin antibody conjugated with horseradish peroxidase in a stabilizing buffer.
4. CHROMOGEN-SUBSTRATE SOLUTION
One vial containing 12 mL of 3,3',5,5'-tetramethybenzidine (TMB) and hydrogen peroxide in citric acid buffer at pH 3.3.
5. STOP SOLUTION
One vial containing 12 ml 0.3 M sulfuric acid.
WARNING: Avoid contact with skin.
6. ANTI-MOUSE PREALBUMIN ELISA MICRO PLATE
Twelve removable eight (8) well micro well strips in well holder frame. Each well is coated with affinity purified anti-Mouse PRE.
7. MOUSE PREALBUMIN STANDARDS
One vial containing a lyophilized Mouse Prealbumin calibrator.
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, plasma and other biological fluids
Sensitivity3.967 ng/ml
Predicted Homology Based on Immunogen SequenceMouse
Publications

Finn, JD et al. A Single Administration of CRISPR/Cas9 Lipid Nanoparticles Achieves Robust and Persistent In Vivo Genome Editing. Cell Rep. 22(9):2227-2235. doi: 10.1016/j.celrep.2018.02.014 (2018). 29490262

Tissue-specific activation of gene expression by the Synergistic Activation Mediator (SAM) CRISPRa system in mice. Nat Commun. 12, 2770 (2021) 33986266

Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Description
Gene SymbolTTR
Gene Full NameTransthyretin
Alias Symbolsprea, D17860, AA408768, AI787086, prealbumin
NCBI Gene Id22139
Protein NameTransthyretin
Description of TargetThis gene encodes a carrier protein responsible for the transport of thyroid hormones and retinol. The protein consists of a tetramer of identical subunits. Due to increased stability of the tetramer form of this encoded protein in mouse, compared to the human protein, this gene product has a reduced tendency to form amyloid fibrils. In humans, this protein binds beta-amyloid preventing its aggregation and providing a neuroprotective role in Alzheimer's disease.
Uniprot IDP07309
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