Prealbumin ELISA Kit (Mouse) (OKIA00111)

Catalog No: OKIA00111 (Formerly GWB-374Z37)

Size:1x96WELLPLATE

Sample Standard Curve is for demonstration only. Do not use for results calculations. A standard curve should be generated each time the test is performed.

Product Info

• Predicted Species Reactivity: Mouse
• Product Format: In Solution
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric, OD 450 nm
• ELISA Kit Duration: 60 Minutes
• ELISA Kit Principle: Aviva Systems Biology Prealbumin ELISA Kit (Mouse) (OKIA00111) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody for Transthyretin has been pre-coated onto a 96-wellplate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and washed to remove unbound proteins. An Anti-Transthyretin - HRP conjugated detector antibody is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Transthyretin captured in the well.
• ELISA Kit Range: 15.6 ng/ml - 500 ng/ml
• ELISA Kit Component:
  • Anti-Mouse Prealbumin (Transthyretin) Microplate: 96 Wells (12 x 8 Well Strips)
  • 5X Diluent Concentrate: 50 mL
  • 20X Wash Solution: 50 mL
  • 100X Enzyme-Antibody Conjugate: 150 uL
  • Chromogen-Substrate Solution: 12 mL
  • Stop Solution: 12 mL
  • Mouse Prealbumin (Transthyretin) Calibrator: 1
• Additional Information: Intended Use: The Prealbumin test kits are a highly sensitive two-site enzyme linked immunoassay (ELISA) for measuring Prealbumin in biological samples of mice.
  Reagents:
  1. DILUENT CONCENTRATE (Running Buffer)
  One bottle containing 50 ml of a 5X concentrated diluent running buffer.
  2. WASH SOLUTION CONCENTRATE
  One bottle containing 50 ml of a 20X concentrated wash solution.
  3. ENZYME-ANTIBODY CONJUGATE 100X
  One vial containing 150 ul of affinity purified anti-Mouse Prealbumin antibody conjugated with horseradish peroxidase in a stabilizing buffer.
  4. CHROMOGEN-SUBSTRATE SOLUTION
  One vial containing 12 mL of 3,3',5,5'-tetramethybenzidine (TMB) and hydrogen peroxide in citric acid buffer at pH 3.3.
  5. STOP SOLUTION
  One vial containing 12 ml 0.3 M sulfuric acid.
  WARNING: Avoid contact with skin.
  6. ANTI-MOUSE PREALBUMIN ELISA MICRO PLATE
  Twelve removable eight (8) well micro well strips in well holder frame. Each well is coated with affinity purified anti-Mouse PRE.
  7. MOUSE PREALBUMIN STANDARDS
  One vial containing a lyophilized Mouse Prealbumin calibrator.
• Reconstitution and Storage: Store as indicated in product manual.
• Sample Type: Serum, plasma and other biological fluids
• Sensitivity: 3.967 ng/ml
• Predicted Homology Based on Immunogen Sequence: Mouse
• Publications:

  Finn, JD et al. A Single Administration of CRISPR/Cas9 Lipid Nanoparticles Achieves Robust and Persistent In Vivo Genome Editing. Cell Rep. 22(9):2227-2235. doi: 10.1016/j.celrep.2018.02.014 (2018). 29490262

  Tissue-specific activation of gene expression by the Synergistic Activation Mediator (SAM) CRISPRa system in mice. Nat Commun. 12, 2770 (2021) 33986266

• Assay Info: Assay Methodology: Quantitative Sandwich ELISA
• Description:
  View product citations for Prealbumin ELISA Kit (Mouse) (OKIA00111) on CiteAb

Target Info

• Gene Symbol: TTR
• Gene Full Name: Transthyretin
• Alias Symbols: prea, D17860, AA408768, AI787086, prealbumin
• NCBI Gene Id: 22139
• Protein Name: Transthyretin
• Description of Target: This gene encodes a carrier protein responsible for the transport of thyroid hormones and retinol. The protein consists of a tetramer of identical subunits. Due to increased stability of the tetramer form of this encoded protein in mouse, compared to the human protein, this gene product has a reduced tendency to form amyloid fibrils. In humans, this protein binds beta-amyloid preventing its aggregation and providing a neuroprotective role in Alzheimer's disease.
• Uniprot ID: P07309