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Catalog No: OKIA00081 (Formerly GWB-246A1C)
Size:1x96WELLPLATE
Price: $417.00
SKU
OKIA00081
Availability: Domestic: within 1 week delivery | International: 1 week
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  • $55: Antibody & Protein in US
  • $55 + $25/Kit in US
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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
COA DatasheetPrintable COA datasheet for OKIA00081
Product Info
Predicted Species ReactivityHuman
Product FormatIn Solution
ApplicationELISA
ELISA Kit Detection MethodColorimetric, OD 450 nm
ELISA Kit Duration100 Minutes
ELISA Kit PrincipleAviva Systems Biology Prealbumin ELISA Kit (Human) (OKIA00081) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody for Transthyretin has been pre-coated onto a 96-wellplate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and washed to remove unbound proteins. An Anti-Transthyretin - HRP conjugated detector antibody is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Transthyretin captured in the well.
ELISA Kit Range3.125 ng/ml - 100 ng/ml
ELISA Kit Component
ComponentAmount
Anti-Human Prealbumin (Transthyretin) Microplate96 Wells (12 x 8 Well Strips)
20X Diluent Concentrate50 mL
20X Wash Solution50 mL
100X Enzyme-Antibody Conjugate150 uL
Chromogen-Substrate Solution12 mL
Stop Solution12 mL
Human Prealbumin (Transthyretin) Calibrator1
Additional InformationIntended Use: The Prealbumin test kits are a highly sensitive two-site enzyme linked immunoassay (ELISA) for measuring Prealbumin in biological samples of human.
::Reagents:
1. DILUENT CONCENTRATE (Running Buffer)
One bottle containing 50 ml of a 5X concentrated diluent running buffer.
2. WASH SOLUTION CONCENTRATE
One bottle containing 50 ml of a 20X concentrated wash solution.
3. ENZYME-ANTIBODY CONJUGATE 100X
One vial containing 150 ul of affinity purified anti-Human Prealbumin antibody conjugated with horseradish peroxidase in a stabilizing buffer.
4. CHROMOGEN-SUBSTRATE SOLUTION
One vial containing 12 mL of 3,3',5,5'-tetramethybenzidine (TMB) and hydrogen peroxide in citric acid buffer at pH 3.3.
5. STOP SOLUTION
One vial containing 12 ml 0.3 M sulfuric acid.
WARNING: Avoid contact with skin.
6. ANTI-HUMAN PREALBUMIN ELISA MICRO PLATE
Twelve removable eight (8) well micro well strips in well holder frame. Each well is coated with affinity purified anti-Human PRE.
7. HUMAN PREALBUMIN STANDARDS
One vial containing a lyophilized Human Prealbumin calibrator.
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, plasma and other biological fluids
Sensitivity0.415 ng/ml
Predicted Homology Based on Immunogen SequenceHuman
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolTTR
Gene Full NameTransthyretin
Alias SymbolsCTS, TTN, ATTR, CTS1, PALB, TBPA, HEL111, HsT2651
NCBI Gene Id7276
Protein NameTransthyretin
Description of TargetThyroid hormone-binding protein. Probably transports thyroxine from the bloodstream to the brain.
Uniprot IDP02766
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