Catalog No: OKCD02821
Size:96WELLS
Price: $700.00
SKU
OKCD02821
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Datasheets/ManualsPrintable datasheet for PRDX6 ELISA Kit (Mouse) (OKCD02821)
Product Info
Predicted Species ReactivityMouse|Mus musculus
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric
ELISA Kit Duration3h
ELISA Kit Linearity
Sample1:21:41:81:16
Serum (n=5)96-105%86-95%84-98%83-92%
EDTA Plasma (n=5)80-91%82-92%91-99%96-105%
Heparin Plasma (n=5)85-94%78-94%90-104%95-104%
ELISA Kit PrincipleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Peroxiredoxin 6 (PRDX6). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Peroxiredoxin 6 (PRDX6). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Peroxiredoxin 6 (PRDX6), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Peroxiredoxin 6 (PRDX6) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
ELISA Kit Range78-5,000pg/mL
ELISA Kit Recovery
MatrixRecovery range (%)Average(%)
Serum (n=5)78-9486
EDTA Plasma (n=5)88-9894
Heparin Plasma(n=5)89-10192
ELISA Kit ReproducibilityIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Peroxiredoxin 6 (PRDX6) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Peroxiredoxin 6 (PRDX6) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
ELISA Kit Component
ComponentAmount
Anti-PRDX6 Microplate96 Wells (12 x 8 Well strips)
PRDX6 Lyophilized Standard2
100X Biotinylated PRDX6 Detector Antibody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
Stop Solution1 x 6 mL
TMB Substrate1 x 9 mL
Reconstitution and Storage2°C to 8°C|-20°C
Sample TypeSerum, plasma, tissue homogenates and other biological fluids
Sensitivity29 pg/mL
SpecificityThis assay has high sensitivity and excellent specificity for detection of Peroxiredoxin 6 (PRDX6). No significant cross-reactivity or interference between Peroxiredoxin 6 (PRDX6) and analogues was observed.
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Protocol Information
Gene SymbolPrdx6
Gene Full Nameperoxiredoxin 6
Alias Symbols1-cys;1-Cys peroxiredoxin;1-Cys Prx;1-cysPrx;9430088D19Rik;a;AA690119;acidic calcium-independent phospholipase A2;aiPLA2;antioxidant protein 2;anti-oxidant protein 2, related sequence 3;Aop2;Brp-;Brp-12;CP-;CP-3;glutathione-dependent peroxiredoxin;GP;GPx;LPC acyltransferase 5;LPCAT-5;Ltw;Ltw-;Ltw4;Ltw-4;Lvtw;Lvtw-4;lyso-PC acyltransferase 5;lysophosphatidylcholine acyltransferase 5;N;non-selenium glutathione peroxidase;NSGPx;ORF06;peroxiredoxin 5, related sequence 3;peroxiredoxin 6, related sequence 3;peroxiredoxin-6;Prdx5.
NCBI Gene Id11758
Protein NamePeroxiredoxin-6
Description of TargetThiol-specific peroxidase that catalyzes the reduction of hydrogen peroxide and organic hydroperoxides to water and alcohols, respectively. Can reduce H(2)O(2) and short chain organic, fatty acid, and phospholipid hydroperoxides. Also has phospholipase activity, and can therefore either reduce the oxidized sn-2 fatty acyl grup of phospholipids (peroxidase activity) or hydrolyze the sn-2 ester bond of phospholipids (phospholipase activity). These activities are dependent on binding to phospholipids at acidic pH and to oxidized phospholipds at cytosolic pH. Plays a role in cell protection against oxidative stress by detoxifying peroxides and in phospholipid homeostasis.
Uniprot IDO08709
Protein Accession #NP_031479.1
Nucleotide Accession #NM_007453.4
Protein Size (# AA)224
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