PRDX4 ELISA Kit (Human) (OKCD08828)

Catalog No: OKCD08828

Size:96WELLS

PRDX4 ELISA Kit (Human) (OKCD08828) Standard Curve

Product Info

• Predicted Species Reactivity: Homo sapiens|Human
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Peroxiredoxin 4 (PRDX4). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Peroxiredoxin 4 (PRDX4). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Peroxiredoxin 4 (PRDX4), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Peroxiredoxin 4 (PRDX4) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 62.5-4,000pg/mL
• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Peroxiredoxin 4 (PRDX4) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Peroxiredoxin 4 (PRDX4) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • PRDX4 Microplate: 96 Wells (12 x 8 Well strips)
  • PRDX4 Lyophilized Standard: 2
  • 100X PRDX4 HRP-Detector Anitbody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 2 mL
  • Stop Solution: 1 x 20 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Serum, plasma and other biological fluids
• Sensitivity: < 13.1pg/mL

Target Info

• Gene Symbol: PRDX4
• Gene Full Name: peroxiredoxin 4
• Alias Symbols: antioxidant enzyme AOE372;AOE372;AOE37-2;epididymis secretory sperm binding protein Li 97n;HEL-S-97n;peroxiredoxin IV;peroxiredoxin-4;PRX-4;prx-IV;thioredoxin peroxidase (antioxidant enzyme);thioredoxin peroxidase AO372;thioredoxin-dependent peroxide reductase A0372;thioredoxin-dependent peroxiredoxin 4.
• NCBI Gene Id: 10549
• Protein Name: Peroxiredoxin-4
• Description of Target: Thiol-specific peroxidase that catalyzes the reduction of hydrogen peroxide and organic hydroperoxides to water and alcohols, respectively. Plays a role in cell protection against oxidative stress by detoxifying peroxides and as sensor of hydrogen peroxide-mediated signaling events. Regulates the activation of NF-kappa-B in the cytosol by a modulation of I-kappa-B-alpha phosphorylation.
• Uniprot ID: Q13162
• Protein Accession #: NP_006397.1
• Nucleotide Accession #: NM_006406.1