Catalog No: OPPA02184 (Formerly GWB-CA8454)
Size:5UG
Price: $75.00
SKU
OPPA02184
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
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- $55: Antibody & Protein in US
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Datasheets/Manuals | Printable datasheet for OPPA02184 |
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Predicted Species Reactivity | Human |
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Product Format | The PRDX3 solution contains 20mM Tris-HCl pH-8, & 10% glycerol. Physical appearance: Sterile Filtered colorless solution. |
Host | E. Coli |
:: | Biological Activity: The specific activity was foun to be approximately 82-83 pmole/min/ ug. The enzymatic activity was confirmed by measuring the remaining peroxide after incubation of PRDX3 and peroxide for 20 min at room temperature. Specific activity is defined as the amount of hydroperoxide that 1ug of enzyme can reduce at 25C for 1 minute. |
:: | Product Introduction: PRDX3 is part of the peroxiredoxin family of antioxidant enzymes, that reduces hydrogen peroxide and alkyl hydroperoxides. PRDX3 is particularly located in the mitochondria and involved in the regulation of cellular redox status by serving as a primary line of defense against H2O2 produced during respiration. PRDX3 is a significant regulator of the abundance of mitochondrial H(2)O(2), which itself promotes apoptosis in cooperation with other mediators of apoptotic signaling. PRDX3 mitochondrial protein is significantly decreased in Alzheimer Disease and Down Syndrome. Product Description: PRDX3 Human Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 195 amino acids (63-256 a.a.) and having a molecular mass of 21.5 kDa.The PRDX3 is purified by proprietary chromatographic techniques. |
Reconstitution and Storage | PRDX3 although stable 4C for 4 weeks, should be stored desiccated below -18C. For long term storage it is recommended to add a carrier protein (0. 1% HSA or BSA). Please prevent freeze-thaw cycles. |
Purity | Greater than 95.0% as determined by SDS-PAGE. |
Peptide Sequence | MPAVTQHAPY FKGTAVVNGE FKDLSLDDFK GKYLVLFFYP LDFTFVCPTE IVAFSDKANE FHDVNCEVVA VSVDSHFSHL AWINTPRKNG GLGHMNIALL SDLTKQISRD YGVLLEGSGL ALRGLFIIDP NGVIKHLSVN DLPVGRSVEE TLRLVKAFQY VETHGEVCPA NWTPDSPTIK PSPAASKEYF QKVNQ. |
Gene Symbol | PRDX3 |
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Alias Symbols | AOP1, MER5, AOP-1, SP-22, HBC189, PRO1748, prx-III |
NCBI Gene Id | 10935 |
Protein Name | Thioredoxin-dependent peroxide reductase, mitochondrial |
Description of Target | Recombinant Human Peroxiredoxin-3 |
Uniprot ID | P30048 |
Protein Accession # | NP_006784.1 |
Protein Size (# AA) | Recombinant |
- Protocol:
- Reconstitution & Storage Instructions
- Western Blotting/Immunoblotting (WB/IB) Protocol
- Immunohistochemistry (IHC) Protocol
- Immunocytochemistry (ICC) Protocol
- Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
- Blocking Peptide Competition Protocol (BPCP)
- Immunoprecipitation (IP) Protocol
- Antibody Array (AA) Protocol
- Tips Information:
-
See our General FAQ page.
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