PRDX3 Polyclonal Antibody (OABB00786)

Catalog No: OABB00786

Size:100UG

Lane :1: Rat Brain Tissue Lysate
Lane 2: Rat Lung Tissue Lysate
Lane 3: Rat Liver Tissue Lysate
Lane 4: Rat Kidney Tissue Lysate
Lane 5: Hela Cell Lysate
Lane 6: Jurkat Cell Lysate
Lane7: 293T Cell Lysate
Lane8:MCF-7 Cell Lysate
Lane9: A549 Cell Lysate

Product Info

• Tested Species Reactivity: Human, Mouse, Rat
• Predicted Species Reactivity: Human|Mouse|Rat
• Product Format: Lyophilized. Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
• Clonality: Polyclonal
• Clone: Polyclonal
• Isotype: Rabbit IgG
• Application: Flow cytometry-intracellular|Immunocytochemistry|Immunofluorescence|Immunohistochemistry|Western blot
• Additional Information: Notes: Tested Species: In-house tested species with positive results.
  Predicted Species: Species predicted to be fit for the product based on sequence similarities.
  By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections.
  Other applications have not been tested.
  Optimal dilutions should be determined by end users.
  
  Background: PRDX3(peroxiredoxin 3) also known as AOP-1, MER5, SP-22 or PRX3, is localized exclusively in mitochondria. The deduced 256-amino acid human AOP1 protein shares 86% amino acid sequence similarity with mouse Aop1, and significant similarity with both the human proliferation-associated gene A product and the mouse stress-induced peritoneal macrophage protein Msp23. The PRDX3 gene is mapped on 10q26.11. Expression of PRDX3 is induced by MYC and is reduced in c-myc -/- cells. Chromatin immunoprecipitation analysis spanning the entire PRDX3 genomic sequence revealed that MYC binds preferentially to a 930-bp region surrounding exon 1. Results using mitochondria-specific fluorescent probes demonstrated that PRDX3 is essential for maintaining mitochondrial mass and membrane potential in transformed rat and human cells. These data provided evidence that PRDX3 is a MYC target gene that is required to maintain normal mitochondrial function.
  Background: PRDX3(peroxiredoxin 3) also known as AOP-1, MER5, SP-22 or PRX3,is localized exclusively in mitochondria. The deduced 256-amino acid human AOP1 protein shares 86% amino acid sequence similarity with mouse Aop1, and significant similarity with both the human proliferation-associated gene A product and the mouse stress-induced peritoneal macrophage protein Msp23. The PRDX3 gene is mapped on 10q26.11. Expression of PRDX3 is induced by MYC and is reduced in c-myc -/- cells. Chromatin immunoprecipitation analysis spanning the entire PRDX3 genomic sequence revealed that MYC binds preferentially to a 930-bp region surrounding exon 1. Results using mitochondria-specific fluorescent probes demonstrated that PRDX3 is essential for maintaining mitochondrial mass and membrane potential in transformed rat and human cells. These data provided evidence that PRDX3 is a MYC target gene that is required to maintain normal mitochondrial function.
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Immunogen: Polypeptide
• Purification: Affinity Purified
• Predicted Homology Based on Immunogen Sequence: Human; Mouse; Rat
• Concentration: 500 ug/ml
• Specificity: No cross reactivity with other proteins.
• Application Info: Western blot: 0.1-0.5 ug/ml: Human, Rat: Mouse
  Immunohistochemistry (Paraffin-embedded Section): 0.5-1 ug/ml: Human, Mouse, Rat: By Heat
  Immunocytochemistry : 0.5-1 ug/ml: Human
• Reference: 1. Chiribau, C. B., Cheng, L., Cucoranu, I. C., Yu, Y.-S., Clempus, R. E., Sorescu, D. FOXO3A
  regulates peroxiredoxin III expression in human cardiac fibroblasts. J. Biol. Chem. 283: 8211-8217,
  2008.
  2. Nemoto, Y., Yamamoto, T., Takada, S., Matsui, Y., Obinata, M. Antisense RNA of the latent period
  gene (MER5) inhibits the differentiation of murine erythroleukemia cells. Gene 91: 261-265, 1990.
  3. Wonsey, D. R., Zeller, K. I., Dang, C. V. The c-Myc target gene PRDX3 is required for mitochondrial
  homeostasis and neoplastic transformation. Proc. Nat. Acad. Sci. 99: 6649-6654, 2002
• Storage Buffer: Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
• Description: Rabbit IgG polyclonal antibody for Thioredoxin-dependent peroxide reductase, mitochondrial(PRDX3) detection. Tested with WB, IHC-P, ICC in Human;Mouse;Rat.

Target Info

• Gene Symbol: PRDX3
• Gene Full Name: peroxiredoxin 3
• Alias Symbols: antioxidant protein 1;AOP1;AOP-1;epididymis secretory sperm binding protein;HBC189;MER5;peroxiredoxin III;Peroxiredoxin-3;PRO1748;protein MER5 homolog;prx-III;SP-22;thioredoxin-dependent peroxide reductase, mitochondrial;thioredoxin-dependent peroxiredoxin 3.
• NCBI Gene Id: 10935
• Protein Name: Thioredoxin-dependent peroxide reductase, mitochondrial
• Description of Target: Thiol-specific peroxidase that catalyzes the reduction of hydrogen peroxide and organic hydroperoxides to water and alcohols, respectively. Plays a role in cell protection against oxidative stress by detoxifying peroxides (PubMed:7733872, PubMed:17707404). Acts synergistically with MAP3K13 to regulate the activation of NF-kappa-B in the cytosol (PubMed:12492477).
• Uniprot ID: P30048
• Molecular Weight: 27693 MW