PRDX3 Antibody - C-terminal region (OAGA03909)

Catalog No: OAGA03909

Size:100 ul

Sample (30 ug of whole cell lysate)
A: 293T
12% SDS PAGE
diluted at 1:1000

Immunohistochemical analysis of paraffin-embedded H358 xenograft, using PRX III antibody at 1:500 dilution.

Peroxiredoxin 3 antibody [C2C3], C-term detects Peroxiredoxin 3 protein at mitochondria by immunofluorescent analysis.
Sample: HeLa cells were fixed in 2% paraformaldehyde/culture medium at 37oC for 30 min.
Green: Peroxiredoxin 3 protein stained by Peroxiredoxin 3 antibody [C2C3], C-term diluted at 1:1000.
Blue: Hoechst 33342 staining.
Scale bar = 10 um.

Product Info

• Predicted Species Reactivity: Human
• Product Format: Liquid
• Clonality: Polyclonal
• Isotype: IgG
• Host: Rabbit
• Application: ICC, IF, IHC-P, WB
• Reconstitution and Storage: Keep as concentrated solution. Aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.
• Immunogen: Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human Peroxiredoxin 3. The exact sequence is proprietary.
• Purification: Purified by antigen-affinity chromatography.
• Concentration: 1 mg/ml
• Preservative: 0.01% Thimerosal
• Application Info: Suggested starting dilutions are as follows: ICC/IF: 1:100-1:1000, IHC-P: 1:100-1:1000, WB: 1:500-1:3000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.
• Storage Buffer: 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Target Info

• Gene Symbol: PRDX3
• Gene Full Name: peroxiredoxin 3
• Alias Symbols: AOP1, MER5, AOP-1, SP-22, HBC189, PRO1748, prx-III
• NCBI Gene Id: 10935
• Protein Name: thioredoxin-dependent peroxide reductase, mitochondrial
• Description of Target: This gene encodes a mitochondrial protein with antioxidant function. The protein is similar to the C22 subunit of Salmonella typhimurium alkylhydroperoxide reductase, and it can rescue bacterial resistance to alkylhydroperoxide in E. coli that lack the C22 subunit. The human and mouse genes are highly conserved, and they map to the regions syntenic between mouse and human chromosomes. Sequence comparisons with recently cloned mammalian homologs suggest that these genes consist of a family that is responsible for the regulation of cellular proliferation, differentiation and antioxidant functions. This family member can protect cells from oxidative stress, and it can promote cell survival in prostate cancer. Alternative splicing of this gene results in multiple transcript variants. Related pseudogenes have been identified on chromosomes 1, 3, 13 and 22.
• Uniprot ID: P30048
• Protein Accession #: NP_001289201.1
• Nucleotide Accession #: NM_001302272.1