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Catalog No: OKCD01780 (Formerly GWB-KBBVH9)
Size:96WELLS
Price: $675.00
SKU
OKCD01780
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Datasheets/ManualsPrintable datasheet for PRB1 ELISA Kit (Human) (OKCD01780)
Product Info
Predicted Species ReactivityHomo sapiens|Human
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric
ELISA Kit Duration3h
ELISA Kit PrincipleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Basic Salivary Proline Rich Protein 1 (PRB1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Basic Salivary Proline Rich Protein 1 (PRB1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Basic Salivary Proline Rich Protein 1 (PRB1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Basic Salivary Proline Rich Protein 1 (PRB1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
ELISA Kit Range1.56-100ng/mL
ELISA Kit ReproducibilityIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Basic Salivary Proline Rich Protein 1 (PRB1) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Basic Salivary Proline Rich Protein 1 (PRB1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
ELISA Kit Component
ComponentAmount
Anti-PRB1 Microplate96 Wells (12 x 8 Well Strips)
PRB1 Lyophilized Standard2 x 100 ng
100X Biotinylated PRB1 Detector Antibody120 uL
Avidin/HRP Conjugate120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Reconstitution and Storage2°C to 8°C|-20°C
Sample TypeSaliva and other biological fluids
Sensitivity< 0.56 ng/mL
SpecificityThis assay has high sensitivity and excellent specificity for detection of Basic Salivary Proline Rich Protein 1 (PRB1). No significant cross-reactivity or interference between Basic Salivary Proline Rich Protein 1 (PRB1) and analogues was observed.
Assay InfoAssay Methodology: Quantitative Sandwich Immunoassay
Gene SymbolPRB1
Gene Full Nameproline rich protein BstNI subfamily 1
Alias Symbolsbasic salivary proline-rich protein 1;BstNI type basic salivary proline-rich protein 1;parotid middle band protein;PM;PMF;PMS;PRB1L;PRB1M;salivary protein Pe.
NCBI Gene Id5542
Protein NameBasic salivary proline-rich protein 1
Description of TargetBasic salivary proline-rich protein 1
Uniprot IDP04280
Protein Accession #NP_005030.2
Nucleotide Accession #NM_005039.3
Protein Size (# AA)392
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