PPY ELISA Kit (Rat) (OKCD07262)

Catalog No: OKCD07262

Size:96WELLS

Product Info

• Predicted Species Reactivity: Rat
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colormetric|Regular detection
• ELISA Kit Duration: 3h
• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Pancreatic Polypeptide (PP). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Pancreatic Polypeptide (PP). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Pancreatic Polypeptide (PP), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Pancreatic Polypeptide (PP) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Pancreatic Polypeptide (PP) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Pancreatic Polypeptide (PP) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • PPY Microplate: 96 Wells (12 x 8 Well strips)
  • PPY Lyophilized Standard: 2
  • 100X PPY HRP-Detector Anitbody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 2 mL
  • Stop Solution: 1 x 20 mL
• Reconstitution and Storage: 2°C to 8°C, -20°C

Target Info

• Gene Symbol: PPY
• Gene Full Name: pancreatic polypeptide
• Alias Symbols: PPY; PNP; Pancreatic Polypeptide Y; Obinepitide; Pancreatic icosapeptide; Pancreatic prohormone
• NCBI Gene Id: 24677
• Protein Name: pancreatic prohormone
• Description of Target: peptide hormone; functions as regulator of pancreatic and astrointestinal functions.
• Uniprot ID: P06303
• Protein Accession #: NP_036758.1
• Nucleotide Accession #: NM_012626.2