PPP4C Antibody (OAAB21650)

Catalog No: OAAB21650

Size:100ul

Western blot detection of Protein Phosphatase 4C in Hela and Jurkat cell lysates using Protein Phosphatase 4C mouse mAb (OAAB21650) (1:200 diluted). Predicted band size: 34KDa. Observed band size: 34KDa.

IHC of paraffin-embedded human breast cancer using anti-Protein Phosphatase 4C mouse mAb (OAAB21650) diluted 1/500-1/1,000.

Immunocytochemistry of HeLa cells using anti-Protein Phosphatase 4C mouse mAb (OAAB21650) diluted 1:200.

Product Info

• Predicted Species Reactivity: Human, Mouse
• Product Format: Liquid
• Clonality: Monoclonal
• Host: Rabbit
• Application: WB, ICC, IHC
• Additional Information: Function: Protein phosphatase that is involved in many processes such as microtubule organization at centrosomes, maturation of spliceosomal snRNPs, apoptosis, DNA repair, tumor necrosis factor (TNF)-alpha signaling, activation of c-Jun N-terminal kinase MAPK8, regulation of histone acetylation, DNA damage checkpoint signaling, NF-kappa-B activation and cell migration. The PPP4C- PPP4R1 PP4 complex may play a role in dephosphorylation and regulation of HDAC3. The PPP4C-PPP4R2-PPP4R3A PP4 complex specifically dephosphorylates H2AFX phosphorylated on Ser-140 (gamma-H2AFX) generated during DNA replication and required for DNA double strand break repair. Dephosphorylates NDEL1 at CDK1 phosphorylation sites and negatively regulates CDK1 activity in interphase (By similarity). In response to DNA damage, catalyzes RPA2 dephosphorylation, an essential step for DNA repair since it allows the efficient RPA2-mediated recruitment of RAD51 to chromatin.
• Reconstitution and Storage: Maintain refrigerated at 2-8C for up to 2 weeks. For long term storage store at -20C in small aliquots to prevent freeze-thaw cycles.
• Predicted Homology Based on Immunogen Sequence: Human, Mouse
• Preservative: 0.02% Sodium Azide and 50% glycerol
• Formulation: Purified mouse monoclonal in PBS (pH 7.4) containing with 0.02% sodium azide and 50% glycerol.
• Application Info: WB: 1:200
  ICC: 1:200
  IHC: 1:100
• Reference: Brewis N.D.,et al.Biochim. Biophys. Acta 1171:231-233(1992).
  Cohen P.T.W.,et al.Submitted (DEC-1998) to the EMBL/GenBank/DDBJ databases.
  Hu M.C.-T.,et al.J. Biol. Chem. 273:33561-33565(1998).
  Zhou G.,et al.J. Biol. Chem. 277:6391-6398(2002).
  Mourtada-Maarabouni M.,et al.Cell Death Differ. 10:1016-1024(2003).
  

Target Info

• Gene Symbol: PPP4C
• Gene Full Name: protein phosphatase 4 catalytic subunit
• Alias Symbols: PP4, PPX, PP-X, PP4C, PPH3, PPP4
• NCBI Gene Id: 5531
• Uniprot ID: P60510
• Protein Accession #: NP_001290432.1
• Nucleotide Accession #: NM_001303503.1
• Molecular Weight: 35 kDa