Catalog No: OOPA00083 (Formerly GWB-BSP291)
Size:5UG
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Datasheets/Manuals | Printable datasheet for OOPA00083 |
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Product Format | Sterile filtered colorless solution. |
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Host | Escherichia Coli. |
:: | Product Introduction: PPP3R1 is a Ser/Thr-specific calcium and calmodulin-dependent protein phosphatase which takes a vital part in the T cell activation pathway. Calcineurin is composed of two subunits; calcineurin A (CnA) and calcineurin B (CnB). Dephosphorylation of the nuclear factor of activated T-cells (NF-AT) by Calcineurin is essential for NF-AT activation, nuclear translocation, and early gene expression in T-cells. Product Description: PPP3R1 produced in E.Coli is a single, non-glycosylated polypeptide chain containing 190 amino acids (1-170.a.a) and having a molecular mass of 21.5kDa. PPP3R1 is fused to a 20 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques. |
Reconstitution and Storage | Store at 4C if entire vial will be used within 2-4 weeks. Store, frozen at -20C for longer periods of time. Please avoid freeze thaw cycles. |
Purity | Greater than 90% as determined by SDS-PAGE. |
Peptide Sequence | MGSSHHHHHH SSGLVPRGSH MGNEASYPLE MCSHFDADEI KRLGKRFKKL DLDNSGSLSV EEFMSLPELQ QNPLVQRVID IFDTDGNGEV DFKEFIEGVS QFSVKGDKEQ KLRFAFRIYD MDKDGYISNG ELFQVLKMMV GNNLKDTQLQ QIVDKTIINA DKDGDGRISF EEFCAVVGGL DIHKKMVVDV |
Formulation | PPP3R1 Human solution (1mg/1ml) containing 20mM Trsi HCL pH-8, 0.1M NaCl, 2mM DTT and 10% glycerol. |
Purification | Greater than 90% as determined by SDS-PAGE. |
Gene Symbol | PPP3R1 |
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Alias Symbols | Protein phosphatase 3 regulatory subunit B alpha, CNB1, CALNB1, Protein phosphatase 3 (formerly 2B) regulatory subunit B (19kD) alpha isoform (calcineurin B type I), Calcineurin B type I (19kDa). |
Protein Size (# AA) | Recombinant |
- Protocol:
- Reconstitution & Storage Instructions
- Western Blotting/Immunoblotting (WB/IB) Protocol
- Immunohistochemistry (IHC) Protocol
- Immunocytochemistry (ICC) Protocol
- Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
- Blocking Peptide Competition Protocol (BPCP)
- Immunoprecipitation (IP) Protocol
- Antibody Array (AA) Protocol
- Tips Information:
-
See our General FAQ page.
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