PPP3R1 ELISA Kit (Mouse) (OKCD02818)

Catalog No: OKCD02818

Size:96WELLS

Mouse PPP3R1 ELISA kit standard curve

Product Info

• Predicted Species Reactivity: Mouse
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colormetric|Regular detection
• ELISA Kit Duration: 3h
• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Protein Phosphatase 3, Regulatory Subunit 1 (PPP3R1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Protein Phosphatase 3, Regulatory Subunit 1 (PPP3R1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Protein Phosphatase 3, Regulatory Subunit 1 (PPP3R1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Protein Phosphatase 3, Regulatory Subunit 1 (PPP3R1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 0.156-10ng/mL
• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Protein Phosphatase 3, Regulatory Subunit 1 (PPP3R1) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Protein Phosphatase 3, Regulatory Subunit 1 (PPP3R1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • Anti-PPP3R1 Microplate: 96 Wells (12 x 8 Well strips)
  • PPP3R1 Lyophilized Standard: 2
  • 100X Biotinylated PPP3R1 Detector Antibody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • Stop Solution: 1 x 6 mL
  • TMB Substrate: 1 x 9 mL
• Reconstitution and Storage: 2°C to 8°C, -20°C
• Sample Type: Tissue homogenates and other biological fluids
• Sensitivity: 0.047 ng/mL
• Specificity: This assay has high sensitivity and excellent specificity for detection of Protein Phosphatase 3, Regulatory Subunit 1 (PPP3R1). No significant cross-reactivity or interference between Protein Phosphatase 3, Regulatory Subunit 1 (PPP3R1) and analogues was observed.
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: PPP3R1
• Gene Full Name: protein phosphatase 3, regulatory subunit B, alpha isoform (calcineurin B, type I)
• Alias Symbols: CaN, Cnb, Cnb1, CaNB1, MCIP1
• NCBI Gene Id: 19058
• Protein Name: Calcineurin subunit B type 1
• Description of Target: Regulatory subunit of calcineurin, a calcium-dependent, calmodulin stimulated protein phosphatase. Confers calcium sensitivity.
• Uniprot ID: Q63810
• Protein Accession #: NP_077779.2
• Nucleotide Accession #: NM_024459.2
• Protein Size (# AA): 170