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Catalog No: OKCD00274 (Formerly GWB-KBBYD8)
Size:96WELLS
Price: $650.00
SKU
OKCD00274
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Datasheets/ManualsPrintable datasheet for PPP3CA ELISA Kit (Human) (OKCD00274)
Product Info
Predicted Species ReactivityHomo sapiens|Human
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric
ELISA Kit Duration3h
ELISA Kit PrincipleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Calcineurin (CaN). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Calcineurin (CaN). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Calcineurin (CaN), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Calcineurin (CaN) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
ELISA Kit Range0.156-10ng/mL
ELISA Kit ReproducibilityIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Calcineurin (CaN) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Calcineurin (CaN) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
ELISA Kit Component
ComponentAmount
Anti-PPP3CA Microplate96 Wells (12 x 8 Well Strips)
PPP3CA Lyophilized Standard2 x 10 ng
100X Biotinylated PPP3CA Detector Antibody120 uL
Avidin/HRP Conjugate120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Reconstitution and Storage2°C to 8°C|-20°C
Sample TypeTissue homogenates, cell lysates and other biological fluids
Sensitivity< 0.057 ng/mL
SpecificityThis assay has high sensitivity and excellent specificity for detection of Calcineurin (CaN). No significant cross-reactivity or interference between Calcineurin (CaN) and analogues was observed.
Assay InfoAssay Methodology: Quantitative Sandwich Immunoassay
Gene SymbolPPP3CA
Gene Full Nameprotein phosphatase 3 catalytic subunit alpha
Alias SymbolsACCIID;calcineurin A alpha;Calmodulin-dependent calcineurin A subunit alpha isoform;CALN;CALNA;CALNA1;CAM-PRP catalytic subunit;CCN1;CNA1;DEE91;IECEE;IECEE1;PPP2B;protein phosphatase 3 catalytic subunit alpha isozyme;serine/threonine-protein phosphatase 2B catalytic subunit alpha isoform.
NCBI Gene Id5530
Protein NameSerine/threonine-protein phosphatase 2B catalytic subunit alpha isoform
Description of TargetCalcium-dependent, calmodulin-stimulated protein phosphatase which plays an essential role in the transduction of intracellular Ca(2+)-mediated signals (PubMed:15671020, PubMed:18838687, PubMed:19154138, PubMed:23468591). Many of the substrates contain a PxIxIT motif and/or a LxVP motif (PubMed:17498738, PubMed:17502104, PubMed:23468591, PubMed:27974827, PubMed:22343722). In response to increased Ca(2+) levels, dephosphorylates and activates phosphatase SSH1 which results in cofilin dephosphorylation (PubMed:15671020). In response to increased Ca(2+) levels following mitochondrial depolarization, dephosphorylates DNM1L inducing DNM1L translocation to the mitochondrion (PubMed:18838687). Dephosphorylates heat shock protein HSPB1 (By similarity). Dephosphorylates and activates transcription factor NFATC1 (PubMed:19154138). In response to increased Ca(2+) levels, regulates NFAT-mediated transcription probably by dephosphorylating NFAT and promoting its nuclear translocation (PubMed:26248042). Dephosphorylates and inactivates transcription factor ELK1 (PubMed:19154138). Dephosphorylates DARPP32 (PubMed:19154138).
Uniprot IDQ08209
Protein Accession #NP_000935
Nucleotide Accession #NM_000944.4
Protein Size (# AA)521
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