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Catalog No: OCOA03284
Size:20UG
Price: $179.00
SKU
OCOA03284
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PPP2R1B Protein Lysate (OCOA03284)

Datasheets/ManualsPrintable datasheet for PPP2R1B Protein Lysate (OCOA03284)
Product Info
Predicted Species ReactivityHomo sapiens
Product FormatLyophilized
HostHEK293T
Additional InformationComponents: 1 vial of 20 ug lyophilized gene specific transient over-expression cell lysate
::Preparation: HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.). To facilitate transportation and protein, the products are supplied as lyophilized proteins.
Reconstitution and StorageThe lysate can be shipped at ambient temperature. Upon receiving, store the sample at -20C. Lysate samples are stable for 12 months from date of receipt when stored at -20C. Avoid repeated freeze-thaw cycles after reconstitution. Lysate samples can be reconstituted with SDS Sample Buffer. Prior to SDS-PAGE fractionation, boil the lysate for 5 minutes
TagC-MYC/DDK
DescriptionPPP2R1B HEK293T cell transient overexpression lysate (as WB positive control)
Gene SymbolPPP2R1B
Gene Full Nameprotein phosphatase 2 scaffold subunit Abeta
Alias SymbolsPP2A subunit A isoform PR65-beta;PP2A subunit A isoform R1-beta;PP2A-Abeta;PR65B;protein phosphatase 2, regulatory subunit A, beta;protein phosphatase 2, structural/regulatory subunit A, beta;serine/threonine-protein phosphatase 2A 65 kDa regulatory subunit A beta isoform.
NCBI Gene Id5519
Protein NameNM_181699, NP_859050, P30154
Description of TargetHomo sapiens protein phosphatase 2 scaffold subunit Abeta (PPP2R1B), transcript variant 2, mRNA, Serine/threonine-protein phosphatase 2A 65 kDa regulatory subunit A beta isoform, serine/threonine-protein phosphatase 2A 65 kDa regulatory subunit A beta isoform isoform b [Homo sapiens]
Uniprot IDhttps://www.uniprot.org/uniprot/P30154
Protein Accession #https://www.ncbi.nlm.nih.gov/protein/NP_859050
Nucleotide Accession #https://www.ncbi.nlm.nih.gov/nuccore/NM_181699
Molecular Weight73.6 kDa
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