PPP1CC Antibody (OAGA00408)

Catalog No: OAGA00408

Size:100 ul

Sample (30 ug of whole cell lysate)
A: Hep G2
B: Molt-4
10% SDS PAGE
OAGA00408 diluted at 1:1000

Sample (30 ug of whole cell lysate)
A: NIH-3T3 10% SDS PAGE
OAGA00408 diluted at 1:1000

Immunohistochemical analysis of paraffin-embedded SAS xenograft, using PPP1CC(OAGA00408) antibody at 1:100 dilution.

Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using PP1 gamma(OAGA00408) antibody at 1:200 dilution.

Product Info

• Predicted Species Reactivity: Human, Mouse
• Product Format: Liquid
• Clonality: Polyclonal
• Isotype: IgG
• Host: Rabbit
• Application: ICC, IF, IHC-FFPE, WB
• Additional Information: Background: Protein phosphatase 1 (PP1) is essential for cell division, and participates in the regulation of glycogen metabolism, muscle contractility and protein synthesis. Involved in regulation of ionic conductances and long-term synaptic plasticity. May play an important role in dephosphorylating substrates such as the postsynaptic density-associated Ca(2+)/calmodulin dependent protein kinase II.
  Positive Controls: 293T, A431, HeLa, HepG2, Molt-4, Raji, NIH-3T3
• Reconstitution and Storage: Keep as concentrated solution. Aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.
• Immunogen: Recombinant fragment corresponding to a region within amino acids 2 and 227 of PP1C gamma (Uniprot ID#P36873)
• Purification: Purified by antigen-affinity chromatography.
• Concentration: 1 mg/ml
• Application Info: Suggested starting dilutions are as follows: WB: 1:500-1:3000, IHC-P: 1:100-1:1000 [suggested antigen retrieval using heat mediated 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0)], ICC/IF: 1:100-1:1000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.
• Storage Buffer: 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Target Info

• Gene Symbol: PPP1CC
• Gene Full Name: Serine/threonine-protein phosphatase PP1-gamma catalytic subunit
• Alias Symbols: PP1C, PP-1G, PPP1G
• NCBI Gene Id: 5501
• Protein Name: Serine/threonine-protein phosphatase PP1-gamma catalytic subunit
• Uniprot ID: P36873
• Protein Accession #: NP_001231903.1
• Molecular Weight: 37 kDa