PPP1CA Antibody (OAAB21633)

Catalog No: OAAB21633

Size:100ul

Western blot detection of PPP1A in HepG2, MCF7 and Hela cell lysates using PPP1A mouse mAb (OAAB21633) (1:1,000 diluted). Predicted band size: 37KDa. Observed band size: 37KDa.

Immunocytochemistry of HeLa cells using anti-PPP1A mouse mAb (OAAB21633) diluted 1:50.

Product Info

• Predicted Species Reactivity: Human, Mouse
• Product Format: Liquid
• Clonality: Monoclonal
• Host: Rabbit
• Application: WB, ICC
• Additional Information: Function: Protein phosphatase that associates with over 200 regulatory proteins to form highly specific holoenzymes which dephosphorylate hundreds of biological targets. Protein phosphatase 1 (PP1) is essential for cell division, and participates in the regulation of glycogen metabolism, muscle contractility and protein synthesis. Involved in regulation of ionic conductances and long-term synaptic plasticity. May play an important role in dephosphorylating substrates such as the postsynaptic density-associated Ca(2+)/calmodulin dependent protein kinase II. Component of the PTW/PP1 phosphatase complex, which plays a role in the control of chromatin structure and cell cycle progression during the transition from mitosis into interphase. Regulates NEK2 function in terms of kinase activity and centrosome number and splitting, both in the presence and absence of radiation-induced DNA damage. Regulator of neural tube and optic fissure closure, and enteric neural crest cell (ENCCs) migration during development. In balance with CSNK1D and CSNK1E, determines the circadian period length, through the regulation of the speed and rhythmicity of PER1 and PER2 phosphorylation. May dephosphorylate CSNK1D and CSNK1E.
• Reconstitution and Storage: Maintain refrigerated at 2-8C for up to 2 weeks. For long term storage store at -20C in small aliquots to prevent freeze-thaw cycles.
• Predicted Homology Based on Immunogen Sequence: Human, Mouse
• Preservative: 0.02% Sodium Azide and 50% glycerol
• Formulation: Purified mouse monoclonal in buffer containing 0.1M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.2% sodium azide, 50% glycerol
• Application Info: WB: 1:1000
  ICC: 1:50
• Reference: Song Q.,et al.Gene 129:291-295(1993).
  Durfee T.,et al.Genes Dev. 7:555-569(1993).
  Tung L.,et al.Submitted (APR-1991) to the EMBL/GenBank/DDBJ databases.
  Ota T.,et al.Nat. Genet. 36:40-45(2004).
  Kalnine N.,et al.Submitted (MAY-2003) to the EMBL/GenBank/DDBJ databases.
  

Target Info

• Gene Symbol: PPP1CA
• Gene Full Name: protein phosphatase 1 catalytic subunit alpha
• Alias Symbols: PP1A, PP-1A, PPP1A, PP1alpha
• NCBI Gene Id: 5499
• Description of Target: The protein encoded by this gene is one of the three catalytic subunits of protein phosphatase 1 (PP1). PP1 is a serine/threonine specific protein phosphatase known to be involved in the regulation of a variety of cellular processes, such as cell division, glycogen metabolism, muscle contractility, protein synthesis, and HIV-1 viral transcription. Increased PP1 activity has been observed in the end stage of heart failure. Studies in both human and mice suggest that PP1 is an important regulator of cardiac function. Mouse studies also suggest that PP1 functions as a suppressor of learning and memory. Three alternatively spliced transcript variants encoding different isoforms have been found for this gene.
• Uniprot ID: P62136
• Protein Accession #: NP_001008709.1
• Nucleotide Accession #: NM_001008709.1
• Molecular Weight: 38 kDa