PPP1CA Antibody - N-terminal region (ARP58652_P050)

Catalog No: ARP58652_P050

WB Suggested Anti-PPP1CA Antibody Titration: 0.2-1 ug/ml
ELISA Titer: 1:1562500
Positive Control: Jurkat cell lysatePPP1CA is supported by BioGPS gene expression data to be expressed in Jurkat

WB Suggested Anti-PPP1CA Antibody
Positive Control: Lane 1: 40ug HEK293 lysate Lane 2: 40ug H1299 lysate
Primary Antibody Dilution : 1:1000
Secondary Antibody : Goat anti-rabbit-HRP
Secondry Antibody Dilution : 1:5000
Submitted by: Jose Luis Rosa, Universitat de Barcelona

Product Info

• Tested Species Reactivity: Human
• Predicted Species Reactivity: Human, Mouse, Rat, Cow, Dog, Guinea Pig, Rabbit
• Product Format: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
• Clonality: Polyclonal
• Host: Rabbit
• Application: WB
• Reconstitution and Storage: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles.
• Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human PPP1CA
• Purification: Affinity Purified
• Predicted Homology Based on Immunogen Sequence: Cow: 100%; Dog: 100%; Guinea Pig: 100%; Human: 100%; Mouse: 100%; Rabbit: 100%; Rat: 100%
• Peptide Sequence: Synthetic peptide located within the following region: MSDSEKLNLDSIIGRLLEGSRVLTPHCAPVQGSRPGKNVQLTENEIRGLC
• Concentration: 0.5 mg/ml
• Blocking Peptide: For anti-PPP1CA (ARP58652_P050) antibody is Catalog # AAP58652 (Previous Catalog # AAPP35789)
• Sample Type Confirmation:

  PPP1CA is supported by BioGPS gene expression data to be expressed in Jurkat

• Reference: Lesage,B., (2007) Biochemistry 46 (31), 8909-8919

Target Info

• Gene Symbol: PPP1CA
• Gene Full Name: Protein phosphatase 1, catalytic subunit, alpha isozyme
• Alias Symbols: PP1A, PP-1A, PPP1A, PP1alpha
• NCBI Gene Id: 5499
• Protein Name: Serine/threonine-protein phosphatase PP1-alpha catalytic subunit
• Description of Target: PPP1CA is one of the three catalytic subunits of protein phosphatase 1 (PP1). PP1 is a serine/threonine specific protein phosphatase known to be involved in the regulation of a variety of cellular processes, such as cell division, glycogen metabolism, muscle contractility, protein synthesis, and HIV-1 viral transcription. Increased PP1 activity has been observed in the end stage of heart failure. Studies in both human and mice suggest that PP1 is an important regulator of cardiac function. Mouse studies also suggest that PP1 functions as a suppressor of learning and memory.The protein encoded by this gene is one of the three catalytic subunits of protein phosphatase 1 (PP1). PP1 is a serine/threonine specific protein phosphatase known to be involved in the regulation of a variety of cellular processes, such as cell division, glycogen metabolism, muscle contractility, protein synthesis, and HIV-1 viral transcription. Increased PP1 activity has been observed in the end stage of heart failure. Studies in both human and mice suggest that PP1 is an important regulator of cardiac function. Mouse studies also suggest that PP1 functions as a suppressor of learning and memory. Three alternatively spliced transcript variants encoding different isoforms have been found for this gene.
• Uniprot ID: Q07161
• Protein Accession #: NP_001008709
• Nucleotide Accession #: NM_001008709
• Protein Size (# AA): 341
• Molecular Weight: 38kDa
• Protein Interactions: HUWE1; PPP1R2P3; CSRNP2; CSRNP1; UBC; SUMO2; SUMO3; BRCA1; Kctd20; WWOX; PKM; SCPEP1; TMOD3; MAT2B; STK24; YWHAH; XPO1; TBCB; CAPNS1; ALDH7A1; ASNS; YAP1; LMTK2; RB1; POLR2A; GSK3B; MST1R; IKBKB; CHUK; CTDP1; TNFRSF1A; tat; PPP1R15A; PPP1R13B; PLCL2; CD2B