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PPME1 ELISA Kit (Rat) (OKEH03629)

96 Wells
$609.00
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Gene Symbol:
PPME1
Official Gene Full Name:
protein phosphatase methylesterase 1
NCBI Gene Id:
361613
Alias Symbols:
PME-1, RGD1309683
Description of Target:
Demethylates proteins that have been reversibly carboxymethylated. Demethylates PPP2CB (in vitro) and PPP2CA. Binding to PPP2CA displaces the manganese ion and inactivates the enzyme.
Protein Name:
Protein phosphatase methylesterase 1
Swissprot Id:
Q4FZT2
Protein Accession #:
NP_001178767.1
Nucleotide Accession #:
NM_001191838.1
Predicted Species Reactivity:
Rat
Sample Type:
Serum, Plasma, Tissue Homogenates, Cell Culture Supernates, Other Biological Fluids
Sensitivity:
0.3125 ng/mL
Kit Range:
0.625 - 40 ng/mL
Kit Duration:
~ 3 Hours
Kit Principle:
Aviva Systems Biology PPME1 ELISA Kit (Rat) (OKEH03629) is based on a competitive enzyme immuno assay technique. The microtiter well-plate in this kit has been pre-coated with an anti-human Ppme1 antibody. Sample or standards are added to the wells along with a fixed quantity of biotinylated Ppme1 and incubated. The Ppme1 found in the sample or standards competes with the biotinylated Ppme1 for limited binding sites on the immobilized anti-human Ppme1 antibody. Excess unbound biotinylated Ppme1 and sample or standard Ppme1 is washed from the plate. Avidin-HRP conjugate is added, incubated and washed. An enzymatic reaction is then produced through the addition of TMB substrate which is catalyzed by the immobilized HRP to generate a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of biotinylated Ppme1 captured in the well and inversely proportional to the amount of Ppme1 which was contained in the sample or standard.
Kit Component:
ComponentAmount
Anti-Ppme1 Microplate96 Wells (12 x 8 Well strips)
Ppme1 Lyophilized Standard2
Sample Diluent1 x 20 mL
100X Ppme1-Biotin Complex1 x 60 uL
100X Avidin-HRP Conjugate1 x 120 uL
Biotin Complex Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Kit Detection Method:
Colorimetric, OD450 nm
Tissue Tool:
Find tissues and cell lines supported by DNA array analysis to express Ppme1.
RNA Seq:
Find tissues and cell lines supported by RNA-seq analysis to express Ppme1.
Datasheets/Manuals:
Click here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Reconstitution and Storage:
Store as indicated in product manual.
Specificity:
This assay recognizes recombinant and natural Rat PME-1. No significant cross-reactivity or interference was observed.
Assay Info:
Assay Methodology: Quantitative Competitive Immunoassay
Molecular Weight:
42 kDa
Protein Size (# AA):
386

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