PPID Antibody - N-terminal region (OAAB05385)

Catalog No: OAAB05385

Size:400UL

Western blot analysis of PPID Antibody (N-term) in NIH-3T3 cell lysates (35ug/lane). PPID (arrow) was detected using the purified Pab.

Western blot analysis of PPID (arrow) using rabbit polyclonal PPID Antibody (N-term) either nontransfected (Lane 1) or lysates transiently transfected transfected (Lane 2) with the PPID gene.

Formalin-fixed and paraffin-embedded human brain tissue reacted with ETFB Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

PPID Antibody (N-term) flow cytometric analysis of k562 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Product Info

• Predicted Species Reactivity: Human|Mouse|Rat
• Product Format: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
• Clonality: Polyclonal
• Isotype: Rabbit Ig
• Host: Rabbit
• Application: FC,IHC-P,WB
• Reconstitution and Storage: Maintain refrigerated at 2-8C for up to 2 weeks. For long term storage store at -20C in small aliquots to prevent freeze-thaw cycles.
• Immunogen: This PPID antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 85-115 amino acids from the N-terminal region of human PPID.
• Predicted Homology Based on Immunogen Sequence: Human; Mouse
• Concentration: Approximately 0.5mg/ml. Actual concentration varies with each lot.
• Application Info: WB: 1:1000
  IHC-P: 1:50~100
  FC: 1:10~50
• Other Applications Image 1 Data: PPID Antibody (N-term) flow cytometric analysis of k562 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Target Info

• Gene Symbol: PPID
• Alias Symbols: 40 kDa peptidyl-prolyl cis-trans isomerase, 40 kDa peptidyl-prolyl cis-trans isomerase D, 4930564J03Rik, cyclophilin 40, cyclophilin D, cyclophilin-40, cyclophilin-related protein, CYP-4, CYP-40, CYPD, cytoplasmic cyclophilin D, P, peptidyl-prolyl cis-trans isomerase D, PPIase D, Ppidl, rotamase D, testicular tissue protein Li 147.
• NCBI Gene Id: 361967|5481|67738
• Protein Name: Peptidyl-prolyl cis-trans isomerase D|peptidyl-prolyl cis-trans isomerase D [Homo sapiens]
• Description of Target: PPIase that catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides and may therefore assist protein folding (PubMed:11350175, PubMed:20676357). Proposed to act as a co-chaperone in HSP90 complexes such as in unligated steroid receptors heterocomplexes. Different co-chaperones seem to compete for association with HSP90 thus establishing distinct HSP90-co-chaperone-receptor complexes with the potential to exert tissue-specific receptor activity control. May have a preference for estrogen receptor complexes and is not found in glucocorticoid receptor complexes. May be involved in cytoplasmic dynein-dependent movement of the receptor from the cytoplasm to the nucleus. May regulate MYB by inhibiting its DNA-binding activity. Involved in regulation of AHR signaling by promoting the formation of the AHR:ARNT dimer; the function is independent of HSP90 but requires the chaperone activity. Involved in regulation of UV radiation-induced apoptosis. Promotes cell viability in anaplastic lymphoma kinase-positive anaplastic large-cell lymphoma (ALK+ ALCL) cell lines.|PPIase that catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides and may therefore assist protein folding. Proposed to act as a co-chaperone in HSP90 complexes such as in unligated steroid receptors heterocomplexes. Different co-chaperones seem to compete for association with HSP90 thus establishing distinct HSP90-co-chaperone-receptor complexes with the potential to exert tissue-specific receptor activity control. May have a preference for estrogen receptor complexes and is not found in glucocorticoid receptor complexes. May be involved in cytoplasmic dynein-dependent movement of the receptor from the cytoplasm to the nucleus. May regulate MYB by inhibiting its DNA-binding activity. Involved in regulation of AHR signaling by promoting the formation of the AHR:ARNT dimer; the function is independent of HSP90 but requires the chaperone activity region. Involved in regulation of UV radiation-induced apoptosis.
• Uniprot ID: Q08752|Q6DGG0|Q9CR16
• Protein Accession #: https://www.ncbi.nlm.nih.gov/protein/NP_005029.1
• Molecular Weight: 40764