PPIA ELISA Kit (Rat) (OKEH04397)

Catalog No: OKEH04397

Size:96T

Rat PPIA ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Rat, Rattus norvegicus
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Duration: ~ 3 Hours
• ELISA Kit Principle: Aviva Systems Biology Ppia ELISA Kit (Rat) (OKEH04397) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Ppia has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. After washing, a biotinylated detector antibody specific for Ppia is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away.An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes to yellow after adding acidicstop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Ppia captured in the well.
• ELISA Kit Range: 0.156-10ng/mL
• ELISA Kit Component:
  • Anti-Ppia Microplate: 96 Wells (12 x 8 Well Strips)
  • Ppia Lyophilized Standard: 2 x 2 ng
  • Sample Diluent: 1 x 20 mL
  • Ppia Biotinylated Detector Antibody: 1 x 120 uL
  • Detector Antibody Diluent: 1 x 12 mL
  • HRP Avidin Conjugate: 1 x 120 uL
  • Conjugate Diluent: 1 x 12 mL
  • 25X Wash Buffer: 1 x 30 mL
  • TMB Substrate: 1 x 10 mL
  • Stop Solution: 1 x 10 mL
• Reconstitution and Storage: Store as indicated in product manual.
• Sample Type: Serum, Plasma, Tissue Homogenates, Cell Culture Supernates, Other Biological Fluids
• Sensitivity: 0.087 ng/mL
• Specificity: Natural and recombinant Rat Peptidyl-prolyl cis-trans isomerase A
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: Ppia, Ppial4d
• Gene Full Name: peptidylprolyl isomerase A, peptidylprolyl isomerase A (cyclophilin A)-like 4D
• Alias Symbols: CYCA;cyclophilin A;cyclosporin A-binding protein;CyP-A;p1B15;p31;peptidyl-prolyl cis-trans isomerase A;PPIase A;rotamase A;TRIMCyp-like.
• NCBI Gene Id: 100360977, 25518
• Protein Name: P10111
• Description of Target: Peptidyl-prolyl cis-trans isomerase A
• Uniprot ID: https://www.uniprot.org/uniprot/P10111
• Protein Accession #: NP_058797.1
• Nucleotide Accession #: NM_017101.1
• Protein Size (# AA): 164
• Molecular Weight: 18 KDa