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Catalog No: OKCD07033
Size:96WELLS
Price: $650.00
SKU
OKCD07033
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Datasheets/ManualsPrintable datasheet for PPIA ELISA Kit (Rat) (OKCD07033)
Product Info
Predicted Species ReactivityRat|Rattus norvegicus
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric
ELISA Kit Duration3h
ELISA Kit PrincipleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cyclophilin A (CYPA). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Cyclophilin A (CYPA). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Cyclophilin A (CYPA), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Cyclophilin A (CYPA) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
ELISA Kit Range1.25-80ng/mL
ELISA Kit ReproducibilityIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Cyclophilin A (CYPA) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Cyclophilin A (CYPA) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
ELISA Kit Component
ComponentAmount
PPIA Microplate96 Wells (12 x 8 Well strips)
PPIA Lyophilized Standard2
100X PPIA HRP-Detector Anitbody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Reconstitution and Storage2°C to 8°C|-20°C
Sample TypeSerum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Sensitivity< 0.47ng/mL
Gene SymbolPpia|Ppial4d
Gene Full Namepeptidylprolyl isomerase A|peptidylprolyl isomerase A (cyclophilin A)-like 4D
Alias SymbolsCYCA;cyclophilin A;cyclosporin A-binding protein;CyP-A;p1B15;p31;peptidyl-prolyl cis-trans isomerase A;PPIase A;rotamase A;TRIMCyp-like.
NCBI Gene Id100360977|25518
Protein NamePeptidyl-prolyl cis-trans isomerase A
Description of TargetPPIases accelerate the folding of proteins. It catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides.
Uniprot IDP10111
Protein Accession #NP_058797.1
Nucleotide Accession #NM_017101.1
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