PPHLN1 Antibody (OAAF02552)

Catalog No: OAAF02552 (Formerly GWB-ASD771)

Size:100 ug

Western blot analysis of extracts from COLO/293 cells, using PPHLN Antibody. The lane on the right is treated with the synthesized peptide.

Immunohistochemistry analysis of paraffin-embedded human liver carcinoma tissue, using PPHLN Antibody. The picture on the right is treated with the synthesized peptide.

Product Info

• Predicted Species Reactivity: Human|Mouse
• Product Format: Liquid
• Clonality: Polyclonal
• Host: Rabbit
• Application: Enzyme-linked immunosorbent assay|Immunofluorescence|Immunohistochemistry|Immunohistochemistry-Paraffin|Western blot
• Reconstitution and Storage: -20°C
• Immunogen: The antiserum was produced against synthesized peptide derived from human PPHLN.
• Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• Specificity: PPHLN antibody detects endogenous levels of total PPHLN protein.
• Formulation: Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Dilution: The optimal working dilution should be determined by the end user.
• Application Info: WB: 1:500~1:3000; IHC: 1:50~1:100; ELISA: 1:5000
• Reference: Line A., Br. J. Cancer 86:1824-1830(2002).
  Kazerounian S., J. Biol. Chem. 278:36707-36717(2003).
  Zhang Q.-H., Genome Res. 10:1546-1560(2000).

Target Info

• Gene Symbol: PPHLN1
• Gene Full Name: periphilin 1
• Alias Symbols: CDC7 expression repressor;CR;gastric cancer antigen Ga50;HSPC206;HSPC232;periphilin-1.
• NCBI Gene Id: 51535
• Protein Name: Periphilin-1
• Description of Target: Component of the HUSH complex, a multiprotein complex that mediates epigenetic repression. The HUSH complex is recruited to genomic loci rich in H3K9me3 and is probably required to maintain transcriptional silencing by promoting recruitment of SETDB1, a histone methyltransferase that mediates further deposition of H3K9me3. In the HUSH complex, contributes to the maintenance of the complex at chromatin (PubMed:26022416). Acts as a transcriptional corepressor and regulates the cell cycle, probably via the HUSH complex (PubMed:15474462, PubMed:17963697). May be involved in epithelial differentiation by contributing to epidermal integrity and barrier formation (Probable).
• Uniprot ID: Q8NEY8
• Protein Accession #: NP_001137259.1
• Molecular Weight: 52 kDa