Catalog No: OKCD02817
Size:96WELLS
Price: $625.00
SKU
OKCD02817
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Datasheets/ManualsPrintable datasheet for PPARG ELISA Kit (Mouse) (OKCD02817)
Product Info
Predicted Species ReactivityMouse|Mus musculus
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric
ELISA Kit Duration3h
ELISA Kit Linearity
Sample1:21:41:81:16
Serum (n=5)80-95%78-105%85-97%94-101%
EDTA Plasma (n=5)88-99%78-92%93-101%93-101%
Heparin Plasma (n=5)78-90%78-89%82-96%91-101%
ELISA Kit PrincipleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Peroxisome Proliferator Activated Receptor Gamma (PPARg). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Peroxisome Proliferator Activated Receptor Gamma (PPARg). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Peroxisome Proliferator Activated Receptor Gamma (PPARg), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Peroxisome Proliferator Activated Receptor Gamma (PPARg) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
ELISA Kit Range0.156-10ng/mL
ELISA Kit Recovery
MatrixRecovery range (%)Average(%)
Serum (n=5)82-9486
EDTA Plasma (n=5)91-9895
Heparin Plasma(n=5)79-9991
ELISA Kit ReproducibilityIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Peroxisome Proliferator Activated Receptor Gamma (PPARg) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Peroxisome Proliferator Activated Receptor Gamma (PPARg) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
ELISA Kit Component
ComponentAmount
Anti-PPARG Microplate96 Wells (12 x 8 Well strips)
PPARG Lyophilized Standard2
100X Biotinylated PPARG Detector Antibody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
Stop Solution1 x 6 mL
TMB Substrate1 x 9 mL
Reconstitution and Storage2°C to 8°C|-20°C
Sample TypeSerum, plasma, tissue homogenates, cell lysates, cell culture cell supernatant and other biological fluids
Sensitivity0.065 ng/mL
SpecificityThis assay has high sensitivity and excellent specificity for detection of Peroxisome Proliferator Activated Receptor Gamma (PPARg). No significant cross-reactivity or interference between Peroxisome Proliferator Activated Receptor Gamma (PPARg) and analogues was observed.
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Protocol Information
Gene SymbolPparg
Gene Full Nameperoxisome proliferator activated receptor gamma
Alias SymbolsNr1;Nr1c3;nuclear receptor subfamily 1 group C member 3;peroxisome proliferator activated receptor gamma 2;peroxisome proliferator activated receptor gamma 4;peroxisome proliferator-activated receptor gamma;PPA;PPAR;Ppar-;PPARgamma;PPAR-gamma;PPARgamma2;PPAR-gamma2.
NCBI Gene Id19016
Protein NamePeroxisome proliferator-activated receptor gamma
Description of TargetNuclear receptor that binds peroxisome proliferators such as hypolipidemic drugs and fatty acids. Once activated by a ligand, the nuclear receptor binds to DNA specific PPAR response elements (PPRE) and modulates the transcription of its target genes, such as acyl-CoA oxidase. It therefore controls the peroxisomal beta-oxidation pathway of fatty acids. Key regulator of adipocyte differentiation and glucose homeostasis. ARF6 acts as a key regulator of the tissue-specific adipocyte P2 (aP2) enhancer. Acts as a critical regulator of gut homeostasis by suppressing NF-kappa-B-mediated proinflammatory responses. Plays a role in the regulation of cardiovascular circadian rhythms by regulating the transcription of ARNTL/BMAL1 in the blood vessels (PubMed:19041764).
Uniprot IDP37238-1
Protein Accession #NP_001120802.1
Nucleotide Accession #NM_001127330.2
Protein Size (# AA)505
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