PPARG ELISA Kit (Bovine) (OKEH03982)

Catalog No: OKEH03982

Size:96T

Bovine PPARG ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Bos taurus, Bovine
• Application: Enzyme-linked Immunosorbent assay-Competitive
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Duration: ~ 3 Hours
• ELISA Kit Principle: Aviva Systems Biology PPARG ELISA Kit (Bovine) (OKEH03982) is based on a competitive enzyme immuno assay technique. The microtiter well-plate in this kit has been pre-coated with an anti-human PPARG antibody. Sample or standards are added to the wells along with a fixed quantity of biotinylated PPARG and incubated. The PPARG found in the sample or standards competes with the biotinylated PPARG for limited binding sites on the immobilized anti-human PPARG antibody. Excess unbound biotinylated PPARG and sample or standard PPARG is washed from the plate. Avidin-HRP conjugate is added, incubated and washed. An enzymatic reaction is then produced through the addition of TMB substrate which is catalyzed by the immobilized HRP to generate a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of biotinylated PPARG captured in the well and inversely proportional to the amount of PPARG which was contained in the sample or standard.
• ELISA Kit Range: 0.78-50ng/mL
• ELISA Kit Component:
  • Anti-PPARG Microplate: 96 Wells (12 x 8 Well strips)
  • PPARG Lyophilized Standard: 2
  • Sample Diluent: 1 x 20 mL
  • 100X PPARG-Biotin Complex: 1 x 60 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Biotin Complex Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 25X Wash Buffer: 1 x 30 mL
  • TMB Substrate: 1 x 10 mL
  • Stop Solution: 1 x 10 mL
• Reconstitution and Storage: Store as indicated in product manual.
• Sample Type: Serum, Plasma, Tissue Homogenates , Cell Culture Supernates, Other Biological Fluids
• Sensitivity: 0.22 ng/mL
• Specificity: Natural and recombinant Bovine Peroxisome proliferator-activated receptor gamma
• Assay Info: Assay Methodology: Quantitative Competitive ELISA

Target Info

• Gene Symbol: PPARG
• Gene Full Name: peroxisome proliferator activated receptor gamma
• Alias Symbols: nuclear receptor subfamily 1 group C member 3;peroxisome proliferative activated receptor, gamma;peroxisome proliferator activated receptor gamma 2;peroxisome proliferator-activated receptor gamma;PPAR-gamma.
• NCBI Gene Id: 281993
• Protein Name: O18971
• Description of Target: Peroxisome proliferator-activated receptor gamma
• Uniprot ID: https://www.uniprot.org/uniprot/O18971
• Protein Accession #: NP_851367.1
• Nucleotide Accession #: NM_181024.2
• Protein Size (# AA): 505
• Molecular Weight: 505 kDa