PPARG Antibody - N- terminal region (OALA01809)

Catalog No: OALA01809 (Formerly GWB-7FA89E)

Size:50 ug

Western blot of affinity purified anti-PPAR2 antibody shows detection of PPAR2 protein in a mouse 3T3 whole cell lysate (lane 1 arrowhead). Approximately 20 ug of lysate was loaded onto a 4-20% gradient gel followed by transfer to nitrocellulose. Primary antibody was used at a 1:2000 dilution in 5% BLOTTO PBS solution. The membrane was washed and reacted with a 1:10000 dilution of IRDye800 Conjugated Affinity Purified Goat-anti-Rabbit IgG [H&L] MX10. Molecular weight estimation was made by comparison to prestained MW markers indicated at the left (lane M). Other detection systems will yield similar results.

Anti-PPARG antibody IHC of human prostate. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody OALA01809 concentration 5 ug/ml.

Product Info

• Tested Species Reactivity: Human
• Predicted Species Reactivity: Human, Macaque
• Product Format: Liquid. Supplied in 0.02 M potassium phosphate, 0.15 M sodium chloride, pH 7.2, 0.01% sodium azide.
• Clonality: Polyclonal
• Isotype: IgG
• Host: Rabbit
• Conjugation: Unconjugated
• Application: ELISA, IHC, IHC-P, WB
• Reconstitution and Storage: Short term: store at 4C. Long term: store at -20C. Avoid freeze-thaw cycles.
• Immunogen: This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to a region near the amino terminus of human PPAR gamma 2.
• Purification: Affinity purified
• Concentration: 1 mg/ml
• Specificity: A BLAST analysis was used to suggest reactivity with this protein from human and macaque based on 100% homology for the immunogen sequence. Cross reactivity with PPARg2 protein from dog and swine is likely due to 94% homology (15/16 identities) with the protein from these sources. Cross reactivity does occur with PPARg2 from mouse. Mouse PPARg2 shows 87% homology to the immunogen. Cross reactivity with PPARg2 homologues from other sources has not been determined. No reactivity is expected against other subtypes of PPAR.
• Epitope: N-Terminus
• Intended Use: For research use only.
• Application Info: ELISA (1:4000 - 1:40000), IHC, IHC-P (2.5 ug/ml), WB (1:1000 - 1:3000)
• Application Data: Immunohistochemistry: OALA01809 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for OALA01809 was determined to be 2.5 ug/ml.
• Storage Buffer: 0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide

Target Info

• Gene Symbol: PPARG
• Alias Symbols: PPARG, NR1C3, PPAR gamma, PPAR gamma 2, PPARG1, PPARG2, PPARgamma, CIMT1, GLM1, PPAR-gamma
• NCBI Gene Id: 5468
• Uniprot ID: P37231
• Protein Accession #: NP_056953.2
• Nucleotide Accession #: NM_015869