PPARG Antibody - N-terminal region (ARP32879_P050)

Catalog No: ARP32879_P050

WB Suggested Anti-PPARG Antibody Titration: 0.2-1 ug/ml
ELISA Titer: 1:500
Positive Control: Jurkat cell lysate

Product Info

• Tested Species Reactivity: Human
• Predicted Species Reactivity: Human, Dog, Pig
• Product Format: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
• Clonality: Polyclonal
• Host: Rabbit
• Application: WB
• Reconstitution and Storage: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles.
• Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human PPARG
• Purification: Affinity Purified
• Predicted Homology Based on Immunogen Sequence: Dog: 92%; Human: 100%; Pig: 85%
• Peptide Sequence: Synthetic peptide located within the following region: MGETLGDSPIDPESDSFTDTLSANISQEMTMVDTEMPFWPTNFGISSVDL
• Concentration: 0.5 mg/ml
• Blocking Peptide: For anti-PPARG (ARP32879_P050) antibody is Catalog # AAP32879 (Previous Catalog # AAPP03903)
• Reference: Jorsal,A., (2008) Mol. Genet. Metab. 94 (3), 347-351

Target Info

• Gene Symbol: PPARG
• Gene Full Name: Peroxisome proliferator-activated receptor gamma
• Alias Symbols: GLM1, CIMT1, NR1C3, PPARG1, PPARG2, PPARG5, PPARgamma
• NCBI Gene Id: 5468
• Protein Name: Peroxisome proliferator-activated receptor gamma
• Description of Target: PPARG is a receptor that binds peroxisome proliferators such as hypolipidemic drugs and fatty acids. Once activated by a ligand, the receptor binds to a promoter element in the gene for acyl-CoA oxidase and activates its transcription. It therefore controls the peroxisomal beta-oxidation pathway of fatty acids. PPARG is the key regulator of adipocyte differentiation and glucose homeostasis.This gene encodes a member of the peroxisome proliferator-activated receptor (PPAR) subfamily of nuclear receptors. PPARs form heterodimers with retinoid X receptors (RXRs) and these heterodimers regulate transcription of various genes. Three subtypes of PPARs are known: PPAR-alpha, PPAR-delta, and PPAR-gamma. The protein encoded by this gene is PPAR-gamma and is a regulator of adipocyte differentiation. Additionally, PPAR-gamma has been implicated in the pathology of numerous diseases including obesity, diabetes, atherosclerosis and cancer. Alternatively spliced transcript variants that encode different isoforms have been described.
• Uniprot ID: P37231
• Protein Accession #: NP_056953
• Nucleotide Accession #: NM_015869
• Protein Size (# AA): 505
• Molecular Weight: 57kDa
• Protein Interactions: HDAC3; NCOA1; NCOA3; SUMO1; UBC; SUMO2; RXRA; MED1; HMGA1; EP300; NCOA2; CDC34; PRKAA2; PRKAA1; PPARGC1A; ASH2L; RBBP5; NSD1; NCOA6; PAXIP1; MUC1; NR3C1; SMAD2; CREBBP; ALOX15B; ZFYVE9; USP7; NCOR1; CDK5; SMARCD3; SMARCA4; NEDD4; BCAS2; MIF4GD; MED14; JAZ