Size:100 ul
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More Information
Predicted Species ReactivityHuman, Mouse, Rat
Product FormatLiquid
ApplicationICC, IF, IHC-FFPE, WB
Additional InformationBackground: The protein encoded by this gene is a member of the inorganic pyrophosphatase (PPase) family. PPases catalyze the hydrolysis of pyrophosphate to inorganic phosphate, which is important for the phosphate metabolism of cells. Studies of a similar protein in bovine suggested a cytoplasmic localization of this enzyme. [provided by RefSeq]
::Positive Controls: H1299, HeLa, HepG2, Molt-4, Neuro 2A, GL261, C8D30, NIH-3T3, BCL-1, Raw264.7, C2C12, PC-12, Rat2
::Cellular Localization: Cytoplasm
Reconstitution and StorageKeep as concentrated solution. Aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.
Replacement ItemThis antibody may replace item sc-100823 from Santa Cruz Biotechnology.
ImmunogenRecombinant fragment corresponding to a region within amino acids 1 and 289 of PPA1 (Uniprot ID#Q15181)
PurificationPurified by antigen-affinity chromatography.
Concentration0.93 mg/ml
Datasheets/ManualsPrintable datasheet for anti-PPA1 antibody - OAGA00880
Application InfoSuggested starting dilutions are as follows: WB: 1:1000-1:10000, IHC-P: 1:100-1:1000 [suggested antigen retrieval using heat mediated 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0)], ICC/IF: 1:100-1:1000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.
Storage Buffer0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
Gene SymbolPPA1
Official Gene Full NameInorganic pyrophosphatase
Alias SymbolsIOPPP antibody, MGC111556 antibody, PP antibody, PP1 antibody, SID6-8061 antibody, PPA1 antibody, pyrophosphate phospho-hydrolase antibody, cytosolic inorganic pyrophosphatase antibody, inorganic pyrophosphatase 1 antibody, inorganic diphosphatase antibod
NCBI Gene Id5464
Protein NameInorganic pyrophosphatase
Swissprot IdQ15181
Protein Accession #NP_066952.1
Molecular Weight33 kDa
Tissue ToolFind tissues and cell lines supported by DNA array analysis to express PPA1.
RNA SeqFind tissues and cell lines supported by RNA-seq analysis to express PPA1.
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