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Datasheets/Manuals | Printable datasheet for PP2A-alpha Antibody (Phospho-Tyr307) (OAAF07429) |
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Predicted Species Reactivity | Human|Mouse|Rat |
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Clonality | Polyclonal |
Host | Rabbit |
Application | Enzyme-linked immunosorbent assay|Immunocytochemistry|Immunofluorescence|Immunohistochemistry|Immunohistochemistry-Paraffin|Western blot |
Additional Information | Modification Sites: Human:Y307 Mouse:Y307 Rat:Y307 |
Reconstitution and Storage | -20°C |
Immunogen | The antiserum was produced against synthesized peptide derived from human PP2A-alpha around the phosphorylation site of Tyr307. |
Purification | The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide. |
Peptide Sequence | Synthetic peptide located within the following region: FSAPNYCYRCGNQAAIMELDDTLKYSFLQFDPAPRRGEPHVTRRTPDYFL |
Concentration | 1mg/ml |
Specificity | PP2A-alpha (Phospho-Tyr307) Antibody detects endogenous levels of PP2A-alpha only when phosphorylated at Tyr307. |
Formulation | Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
Application Info | WB: 1:500~1:1000 IHC: 1:50~1:100 IF: 1:100~1:500 ELISA: 1:5000 |
Gene Symbol | PPP2CA |
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Gene Full Name | protein phosphatase 2 catalytic subunit alpha |
Alias Symbols | NEDLBA;PP2A-alpha;PP2Ac;PP2CA;PP2Calpha;protein phosphatase 2, catalytic subunit, alpha isozyme;replication protein C;RP-C;serine/threonine-protein phosphatase 2A catalytic subunit alpha isoform. |
NCBI Gene Id | 5515 |
Protein Name | Serine/threonine-protein phosphatase 2A catalytic subunit alpha isoform |
Description of Target | PP2A is the major phosphatase for microtubule-associated proteins (MAPs). PP2A can modulate the activity of phosphorylase B kinase casein kinase 2, mitogen-stimulated S6 kinase, and MAP-2 kinase. Cooperates with SGO2 to protect centromeric cohesin from separase-mediated cleavage in oocytes specifically during meiosis I (By similarity). Can dephosphorylate SV40 large T antigen and p53/TP53. Activates RAF1 by dephosphorylating it at 'Ser-259'. |
Uniprot ID | P67775 |
Molecular Weight | 35 kDa |
- Protocol:
- Reconstitution & Storage Instructions
- Western Blotting/Immunoblotting (WB/IB) Protocol
- Immunohistochemistry (IHC) Protocol
- Immunocytochemistry (ICC) Protocol
- Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
- Blocking Peptide Competition Protocol (BPCP)
- Immunoprecipitation (IP) Protocol
- Antibody Array (AA) Protocol
- Tips Information:
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See our General FAQ page.
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What is the species homology for "PP2A-alpha Antibody (Phospho-Tyr307) (OAAF07429)"?
The tested species reactivity for this item is "". This antibody is predicted to have homology to "Human|Mouse|Rat".
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How long will it take to receive "PP2A-alpha Antibody (Phospho-Tyr307) (OAAF07429)"?
This item is available "Domestic: within 1-2 week delivery | International: 1-2 weeks".
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What buffer format is "PP2A-alpha Antibody (Phospho-Tyr307) (OAAF07429)" provided in?
This item is provided in "".
Additional format options may be available. For more information please contact info@avivasysbio.com. -
What are other names for "PP2A-alpha Antibody (Phospho-Tyr307) (OAAF07429)"?
This target may also be called "NEDLBA;PP2A-alpha;PP2Ac;PP2CA;PP2Calpha;protein phosphatase 2, catalytic subunit, alpha isozyme;replication protein C;RP-C;serine/threonine-protein phosphatase 2A catalytic subunit alpha isoform." in publications.
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What is the shipping cost for "PP2A-alpha Antibody (Phospho-Tyr307) (OAAF07429)"?
The shipping cost for this item is $40 within the US. Please contact us for specific shipping prices for international orders.
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What is the guarantee for "PP2A-alpha Antibody (Phospho-Tyr307) (OAAF07429)"?
All Aviva products have been through rigorous validations and carry 100% satisfaction guarantee.
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Can I get bulk pricing for "PP2A-alpha Antibody (Phospho-Tyr307) (OAAF07429)"?
You can get bulk pricing for this item by going here.
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What is the molecular weight of the protein?
The molecular weight reported by Uniprot for this item is "35 kDa".
Please note observed molecular weights in western blot applications may differ depending on a variety of protein characteristics. -
What protocols are available for "PP2A-alpha Antibody (Phospho-Tyr307) (OAAF07429)"?
We may have detailed protocol data avaialble for this item. To learn more, please view the "Protocols & Data" tab on the product page.
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What are positive controls for "PPP2CA"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
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What are negative controls for "PPP2CA"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
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What other proteins interact with "PPP2CA"?
This protein has been reported to interact with "Protein Interactions". Please view the "Related Categories" tab on the product page for more information.
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What biological processes are associated with "PPP2CA"?
This protein has been associated with "Biological Processes". Please view the "Related Categories" tab on the product page for more information.
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What cellular components are associated with "PPP2CA"?
This protein has been associated with "Cellular Components". Please view the "Related Categories" tab on the product page for more information.
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What protein functions are associated with "PPP2CA"?
This protein has been associated with "Protein Functions". Please view the "Related Categories" tab on the product page for more information.