Catalog No: OKCD06333
Size:96WELLS
Price: $625.00
SKU
OKCD06333
Availability: Domestic: within 1-2 weeks delivery | International: within 1-2 weeks delivery
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Datasheets/Manuals | Printable datasheet for POU5F1 ELISA Kit (Mouse) (OKCD06333) |
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Predicted Species Reactivity | Mouse|Mus musculus | ||||||||||||||||||||||
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Application | Enzyme-linked Immunosorbent assay-Sandwich | ||||||||||||||||||||||
ELISA Kit Detection Method | Colorimetric | ||||||||||||||||||||||
ELISA Kit Duration | 3h | ||||||||||||||||||||||
ELISA Kit Principle | The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Octamer Binding Transcription Factor 4 (OCT4). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Octamer Binding Transcription Factor 4 (OCT4). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Octamer Binding Transcription Factor 4 (OCT4), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Octamer Binding Transcription Factor 4 (OCT4) in the samples is then determined by comparing the O.D. of the samples to the standard curve. | ||||||||||||||||||||||
ELISA Kit Range | 0.156-10ng/mL | ||||||||||||||||||||||
ELISA Kit Reproducibility | Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Octamer Binding Transcription Factor 4 (OCT4) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Octamer Binding Transcription Factor 4 (OCT4) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12% | ||||||||||||||||||||||
ELISA Kit Component |
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Reconstitution and Storage | 2°C to 8°C|-20°C | ||||||||||||||||||||||
Sample Type | Tissue homogenates and other biological fluids. | ||||||||||||||||||||||
Sensitivity | < 0.057ng/mL |
Gene Symbol | Pou5f1 |
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Gene Full Name | POU domain, class 5, transcription factor 1 |
Alias Symbols | NF-A3;Oct;Oct-;Oct3;Oct-3;Oct3/;Oct-3/;Oct3/4;Oct-3/4;Oct4;Oct-4;octamer-binding protein 3;octamer-binding protein 4;octamer-binding transcription factor 3;octamer-binding transcription factor 4 alternative;Otf;Otf-;Otf3;Otf3-;Otf-3;Otf3g;Otf3-rs7;Otf4;Otf-4;POU domain class 5 transcription factor 1 transcript variant 2 oct4b';POU domain class 5 transcription factor 1 transcript variant 3 oct4b;POU domain, class 5, transcription factor 1. |
NCBI Gene Id | 18999 |
Protein Name | POU domain, class 5, transcription factor 1 |
Description of Target | Transcription factor that binds to the octamer motif (5'-ATTTGCAT-3') (PubMed:1972777, PubMed:1690859, PubMed:1967980, PubMed:17525163, PubMed:23376973). Forms a trimeric complex with SOX2 on DNA and controls the expression of a number of genes involved in embryonic development such as YES1, FGF4, UTF1 and ZFP206 (PubMed:17097055, PubMed:17496161, PubMed:19740739). Critical for early embryogenesis and for embryonic stem cell pluripotency (PubMed:1972777, PubMed:1690859, PubMed:17496161, PubMed:18662995, PubMed:19740739, PubMed:29153991, PubMed:23376973). |
Uniprot ID | P20263 |
Protein Accession # | NP_038661.2 |
Nucleotide Accession # | NM_013633.3 |
- Protocol:
- Reconstitution & Storage Instructions
- Western Blotting/Immunoblotting (WB/IB) Protocol
- Immunohistochemistry (IHC) Protocol
- Immunocytochemistry (ICC) Protocol
- Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
- Blocking Peptide Competition Protocol (BPCP)
- Immunoprecipitation (IP) Protocol
- Antibody Array (AA) Protocol
- Tips Information:
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See our General FAQ page.
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