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Catalog No: OKCD06333
Size:96WELLS
Price: $625.00
SKU
OKCD06333
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Datasheets/ManualsPrintable datasheet for POU5F1 ELISA Kit (Mouse) (OKCD06333)
Product Info
Predicted Species ReactivityMouse|Mus musculus
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric
ELISA Kit Duration3h
ELISA Kit PrincipleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Octamer Binding Transcription Factor 4 (OCT4). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Octamer Binding Transcription Factor 4 (OCT4). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Octamer Binding Transcription Factor 4 (OCT4), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Octamer Binding Transcription Factor 4 (OCT4) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
ELISA Kit Range0.156-10ng/mL
ELISA Kit ReproducibilityIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Octamer Binding Transcription Factor 4 (OCT4) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Octamer Binding Transcription Factor 4 (OCT4) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
ELISA Kit Component
ComponentAmount
POU5F1 Microplate96 Wells (12 x 8 Well strips)
POU5F1 Lyophilized Standard2
100X POU5F1 HRP-Detector Anitbody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Reconstitution and Storage2°C to 8°C|-20°C
Sample TypeTissue homogenates and other biological fluids.
Sensitivity< 0.057ng/mL
Gene SymbolPou5f1
Gene Full NamePOU domain, class 5, transcription factor 1
Alias SymbolsNF-A3;Oct;Oct-;Oct3;Oct-3;Oct3/;Oct-3/;Oct3/4;Oct-3/4;Oct4;Oct-4;octamer-binding protein 3;octamer-binding protein 4;octamer-binding transcription factor 3;octamer-binding transcription factor 4 alternative;Otf;Otf-;Otf3;Otf3-;Otf-3;Otf3g;Otf3-rs7;Otf4;Otf-4;POU domain class 5 transcription factor 1 transcript variant 2 oct4b';POU domain class 5 transcription factor 1 transcript variant 3 oct4b;POU domain, class 5, transcription factor 1.
NCBI Gene Id18999
Protein NamePOU domain, class 5, transcription factor 1
Description of TargetTranscription factor that binds to the octamer motif (5'-ATTTGCAT-3') (PubMed:1972777, PubMed:1690859, PubMed:1967980, PubMed:17525163, PubMed:23376973). Forms a trimeric complex with SOX2 on DNA and controls the expression of a number of genes involved in embryonic development such as YES1, FGF4, UTF1 and ZFP206 (PubMed:17097055, PubMed:17496161, PubMed:19740739). Critical for early embryogenesis and for embryonic stem cell pluripotency (PubMed:1972777, PubMed:1690859, PubMed:17496161, PubMed:18662995, PubMed:19740739, PubMed:29153991, PubMed:23376973).
Uniprot IDP20263
Protein Accession #NP_038661.2
Nucleotide Accession #NM_013633.3
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