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POU5F1 Antibody (OAGA01617)

100 ul
$409.00
In Stock
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Gene Symbol:
POU5F1
Official Gene Full Name:
POU class 5 homeobox 1
NCBI Gene Id:
5460
Swissprot Id:
Q01860
Alias Symbols:
OCT3, OCT4, OTF3, OTF4, OTF-3, Oct-3, Oct-4
Replacement Item:
This antibody may replace item sc-101534 from Santa Cruz Biotechnology.
Description of Target:
This gene encodes a transcription factor containing a POU homeodomain that plays a key role in embryonic development and stem cell pluripotency. Aberrant expression of this gene in adult tissues is associated with tumorigenesis. This gene can participate in a translocation with the Ewing's sarcoma gene on chromosome 21, which also leads to tumor formation. Alternative splicing, as well as usage of alternative AUG and non-AUG translation initiation codons, results in multiple isoforms. One of the AUG start codons is polymorphic in human populations. Related pseudogenes have been identified on chromosomes 1, 3, 8, 10, and 12.
Host:
Rabbit
Clonality:
Polyclonal
Purification:
Purified by antigen-affinity chromatography.
Application:
FACS, IF, IHC-P, IP, WB
Tissue Tool:
Find tissues and cell lines supported to express POU5F1.
RNA Seq:
Find tissues and cell lines supported by RNA-seq analysis to express POU5F1.
Immunogen:
Recombinant protein encompassing a sequence within the center region of human Oct4. The exact sequence is proprietary.
Predicted Species Reactivity:
Human, Mouse
Product Format:
Liquid. 1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.
Reconstitution and Storage:
Keep as concentrated solution. Aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.
Concentration:
1mg/ml
Datasheets/Manuals:
Printable datasheet for anti-POU5F1 (OAGA01617) antibody
Isotype:
IgG
Application Info:
Suggested starting dilutions are as follows: WB: 1:5000-1:50000, IHC-P: 1:100-1:1000 [suggested antigen retrieval using heat mediated 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0)], ICC/IF: 1:100-1:1000, IP: 1:500-1:1000, FACS: 1:50-1:200. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.

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