POU4F1 Antibody (OABF01290)

Catalog No: OABF01290

Size:100UL

Lane 1: rat brain lysates Lane 2: human colon carcinoma lysates probed with Anti BRN3A Polyclonal Antibody, Unconjugated (OABF01290) at 1:200 in 4C. Followed by conjugation to secondary antibody at 1:3000 90min in 37C. Predicted band 43kD. Observed band size: 43kD.

Product Info

• Tested Species Reactivity: Human, Mouse, Rat
• Predicted Species Reactivity: Bovine|Chicken|Dog|Human|Mouse|Porcine|Rabbit|Rat
• Product Format: Liquid.Aqueous buffered solution containing 1% BSA, 50% glycerol and 0.09% sodium azide.
• Clonality: Polyclonal
• Isotype: IgG
• Host: Rabbit
• Conjugation: Unconjugated
• Application: Enzyme-linked immunosorbent assay|Flow cytometry|Immunocytochemistry|Immunofluorescence|Immunohistochemistry-Frozen|Immunohistochemistry-Paraffin|Western blot
• Additional Information: Subcellular location: Nucleus
• Reconstitution and Storage: -20°C
• Immunogen: KLH conjugated synthetic peptide derived from human BRN3A
• Purification: Protein A Purified
• Concentration: 1 ug/ul
• Application Info: WB: 1:300 - 1,000
  IHC-P: 1:200 - 400
  IHC-F: 1:50-200
  ELISA: 1:500-1000
  FC: 1:20-100
  Fluorescent (IHC-P): 1:50-200
  Fluorescent (IHC-F): 1:50-200
  Fluorescent (ICC): 1:50-200
  Optimal dilutions should be determined by the end user.

Target Info

• Gene Symbol: POU4F1
• Gene Full Name: POU class 4 homeobox 1
• Alias Symbols: ATITHS;brain-specific homeobox/POU domain protein 3A;brn-3A;BRN3A;homeobox/POU domain protein RDC-1;Oct-T1;POU domain, class 4, transcription factor 1;RDC-1.
• NCBI Gene Id: 5457
• Protein Name: POU domain, class 4, transcription factor 1
• Description of Target: Multifunctional transcription factor with different regions mediating its different effects. Acts by binding (via its C-terminal domain) to sequences related to the consensus octamer motif 5'-ATGCAAAT-3' in the regulatory regions of its target genes. Regulates the expression of specific genes involved in differentiation and survival within a subset of neuronal lineages. It has been shown that activation of some of these genes requires its N-terminal domain, maybe through a neuronal-specific cofactor. Ativates BCL2 expression and protects neuronal cells from apoptosis (via the N-terminal domain). Induces neuronal process outgrowth and the coordinate expression of genes encoding synaptic proteins. Exerts its major developmental effects in somatosensory neurons and in brainstem nuclei involved in motor control. Stimulates the binding affinity of the nuclear estrogene receptor ESR1 to DNA estrogen response element (ERE), and hence modulates ESR1-induced transcriptional activity. May positively regulate POU4F2 and POU4F3. Regulates dorsal root ganglion sensory neuron specification and axonal projection into the spinal cord. Plays a role in TNFSF11-mediated terminal osteoclast differentiation. Negatively regulates its own expression interacting directly with a highly conserved autoregulatory domain surrounding the transcription initiation site.
• Uniprot ID: Q01851