POU3F3 ELISA Kit (Human) (OKEH08392)

Catalog No: OKEH08392

Size:96T

Product Info

• Predicted Species Reactivity: Homo sapiens, Human
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Principle: Aviva Systems Biology POU3F3 ELISA Kit (Human) (OKEH08392) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for POU3F3 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for POU3F3 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample POU3F3 captured in well.
• ELISA Kit Range: 62.5-4000pg/mL
• ELISA Kit Recovery: Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 99% (n = 20)
• ELISA Kit Reproducibility: Mean Intra-assay CV%: <=8.8% (n = 20)
  Mean Inter-assay CV%: <=12.3% (n = 20)
• ELISA Kit Component:
  • POU3F3 Microplate: 96 Wells (12 x 8 Well strips)
  • POU3F3 Lyophilized Standard: 2
  • 100X Biotinylated POU3F3 Detector Antibody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Sample Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 25X Wash Buffer: 1 x 30 mL
  • TMB Substrate: 1 x 10 mL
  • Stop Solution: 1 x 10 mL
• Additional Information: Function: Transcription factor that acts synergistically with SOX11 and SOX4. Plays a role in neuronal development. Is implicated in an enhancer activity at the embryonic met-mesencephalic junction; the enhancer element contains the octamer motif (5'-ATTTGCAT-3').
  Subcellular Location: Nucleus
  Unigene: Hs.248158
  SMR: P20264
  String: 9606.ENSP00000355001
  KEGG: hsa:5455
  MIM: 602480
  Pfam: PF00046
• Reconstitution and Storage: Store as indicated in product manual.
• Sensitivity: 31.25pg/mL
• Specificity: Natural and recombinant Human POU domain, class 3, transcription factor 3
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: POU3F3
• Gene Full Name: POU class 3 homeobox 3
• Alias Symbols: brain-1;brain-specific homeobox/POU domain protein 1;BRN1;oct-8;octamer-binding protein 8;octamer-binding transcription factor 8;OTF8;POU domain, class 3, transcription factor 3;SNIBFIS.
• NCBI Gene Id: 5455
• Protein Name: P20264
• Description of Target: POU domain, class 3, transcription factor 3
• Uniprot ID: https://www.uniprot.org/uniprot/P20264
• Protein Accession #: NP_006227.1
• Nucleotide Accession #: NM_006236.2