POU3F2 Antibody - middle region (OAAB12218)

Catalog No: OAAB12218

Size:400ul

POU3F2 Antibody (Center) (Cat. # OAAB12218) western blot analysis in K562 cell line lysates (35ug/lane).This demonstrates the POU3F2 antibody detected the POU3F2 protein (arrow).

Product Info

• Predicted Species Reactivity: Human
• Product Format: Liquid. PBS with 0.09% (W/V) sodium azide.
• Clonality: Polyclonal
• Isotype: Ig
• Host: Rabbit
• Application: WB
  Function: Transcription factor that binds preferentially to the recognition sequence which consists of two distinct half-sites, ('GCAT') and ('TAAT'), separated by a nonconserved spacer region of 0, 2, or 3 nucleotides. Positively regulates the genes under the control of corticotropin-releasing hormone (CRH) and CRH II promoters (By similarity).
  Cellular Location: Nucleus.
  Tissue Location: Expressed specifically in the neuroectodermal cell lineage.
• Reconstitution and Storage: Maintain refrigerated at 2-8C for up to 2 weeks. For long term storage store at -20C in small aliquots to prevent freeze-thaw cycles.
• Immunogen: This POU3F2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 199-226 amino acids from the Central region of human POU3F2.
• Purification: This antibody is purified through a protein A column, followed by peptide affinity purification.
• Predicted Homology Based on Immunogen Sequence: Mouse, Rat
• Concentration: Approximately 0.5mg/ml. Actual concentration varies with each lot.
• Application Info: WB: 1:1000

Target Info

• Gene Symbol: POU3F2
• Alias Symbols: BRN2, OCT7, OTF7, OTF-7, POUF3, brn-2, oct-7, N-Oct3
• NCBI Gene Id: 5454
• Protein Name: POU domain, class 3, transcription factor 2
• Description of Target: POU3F2 belongs to a large family of transcription factors that bind to the octameric DNA sequence ATGCAAAT. Most of these proteins share a highly homologous region, referred to as the POU domain, that occurs in several mammalian transcription factors, including the octamer-binding proteins Oct1 (POU2F1; MIM 164175) and Oct2 (POU2F2; MIM 164176) and the pituitary protein Pit1 (PIT1; MIM 173110). Class III POU genes are expressed predominantly in the central nervous system (CNS). It is likely that CNS-specific transcription factors such as these play an important role in mammalian neurogenesis by regulating their diverse patterns of gene [removed]Schreiber et al., 1993 [PubMed 8441633]; Atanasoski et al., 1995 [PubMed 7601453]).
• Uniprot ID: P20265
• Protein Accession #: NP_005595.2
• Molecular Weight: 47 kDa