POU2F3 ELISA Kit (Human) (OKEH08260)

Catalog No: OKEH08260

Size:96T

Product Info

• Predicted Species Reactivity: Homo sapiens, Human
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Principle: Aviva Systems Biology POU2F3 ELISA Kit (Human) (OKEH08260) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for POU2F3 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for POU2F3 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample POU2F3 captured in well.
• ELISA Kit Range: 0.312-20ng/mL
• ELISA Kit Recovery: Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 88% (n = 20)
• ELISA Kit Reproducibility: Mean Intra-assay CV%: <=7.2% (n = 20)
  Mean Inter-assay CV%: <=8.6% (n = 20)
• ELISA Kit Component:
  • POU2F3 Microplate: 96 Wells (12 x 8 Well strips)
  • POU2F3 Lyophilized Standard: 2
  • 100X Biotinylated POU2F3 Detector Antibody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Sample Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 25X Wash Buffer: 1 x 30 mL
  • TMB Substrate: 1 x 10 mL
  • Stop Solution: 1 x 10 mL
• Additional Information: Function: Transcription factor that binds to the octamer motif (5'-ATTTGCAT-3'). Regulated the expression of a number of genes such as SPRR2A or placental lactogen.
  Subcellular Location: Nucleus
  Unigene: Hs.227115
  SMR: Q9UKI9
  String: 9606.ENSP00000260264
  KEGG: hsa:25833
  MIM: 607394
  Pfam: PF00046
• Reconstitution and Storage: Store as indicated in product manual.
• Sensitivity: 0.169ng/mL
• Specificity: Natural and recombinant Human POU domain, class 2, transcription factor 3
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: POU2F3
• Gene Full Name: POU class 2 homeobox 3
• Alias Symbols: Epoc-1;OCT11;OCT-11;octamer-binding protein 11;octamer-binding transcription factor 11;OTF-11;PLA1;PLA-1;POU domain transcription factor OCT11a;POU domain, class 2, transcription factor 3;Skn-1a;transcription factor PLA-1;transcription factor Skn-1.
• NCBI Gene Id: 25833
• Protein Name: Q9UKI9
• Description of Target: POU domain, class 2, transcription factor 3
• Uniprot ID: https://www.uniprot.org/uniprot/Q9UKI9
• Protein Accession #: NP_001231611.1
• Nucleotide Accession #: NM_001244682.1