Catalog No: OKEH04220
Size:96T
Price: $525.00
SKU
OKEH04220
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityHomo sapiens, Human
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~ 3 Hours
ELISA Kit PrincipleAviva Systems Biology POU2F2 ELISA Kit (Human) (OKEH04220) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for POU2F2 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for POU2F2 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample POU2F2 captured in the well.
ELISA Kit Range31.2-2000pg/mL
ELISA Kit RecoveryMean recovery when spiking into sample matrices at concentrations within the dynamic range: 85%
ELISA Kit ReproducibilityMean Intra-assay CV%: <= 4.5% (n = 20)
Mean Inter-assay CV%: <= 8.4% (n = 20)
ELISA Kit Component
ComponentAmount
POU2F2 Microplate96 Wells (12 x 8 Well Strips)
POU2F2 Standard2 x 2 ng
Sample Diluent1 x 20 mL
POU2F2 Biotinylated Detector Antibody1 x 120 uL
Detector Antibody Diluent1 x 12 mL
HRP Avidin Conjugate1 x 120 uL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Tissue homogenates, Cell culture supernates and Other biological fluids.
Sensitivity10 pg/mL
SpecificityNatural and recombinant Human POU domain, class 2, transcription factor 2
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolPOU2F2
Gene Full NamePOU class 2 homeobox 2
Alias Symbolshomeobox protein;lymphoid-restricted immunoglobulin octamer-binding protein NF-A2;Oct-2;OCT2;octamer-binding protein 2;octamer-binding transcription factor 2;OTF2;POU domain, class 2, transcription factor 2.
NCBI Gene Id5452
Protein NameP09086
Description of TargetPOU domain, class 2, transcription factor 2
Uniprot IDhttps://www.uniprot.org/uniprot/P09086
Protein Accession #NP_001193954.1
Nucleotide Accession #NM_001207025.2
Protein Size (# AA)479
Molecular Weight51 kDa
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