POTEG Chemi-Luminescent ELISA Kit (Human) (OKCD03888)

Catalog No: OKCD03888

Size:96WELLS

Human POTEG ELISA Kit Standard Curve

Product Info

• Predicted Species Reactivity: Homo sapiens|Human
• Application: Chemiluminescent immunoassay
• ELISA Kit Detection Method: Chemiluminescent
• ELISA Kit Duration: 2h, 40min
• ELISA Kit Linearity:

  Sample	1:2	1:4	1:8	1:16
  Serum (n=5)	88-102%	86-95%	89-103%	95-102%
  EDTA Plasma (n=5)	78-90%	99-105%	80-95%	89-101%
  Heparin Plasma (n=5)	97-104%	81-88%	89-97%	95-103%

• ELISA Kit Principle: The microplate provided in this kit has been pre-coated with an antibody specific to POTE Ankyrin Domain Family, Member G (POTEG). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to POTE Ankyrin Domain Family, Member G (POTEG). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the POTE Ankyrin Domain Family, Member G (POTEG) level in the sample or standard.
• ELISA Kit Range: 27.4-20,000pg/mL
• ELISA Kit Recovery:

  Matrix	Recovery range (%)	Average(%)
  Serum (n=5)	79-88	83
  EDTA Plasma (n=5)	95-103	99
  Heparin Plasma (n=5)	88-102	93

• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level POTE Ankyrin Domain Family, Member G (POTEG) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level POTE Ankyrin Domain Family, Member G (POTEG) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • POTEG Microplate: 96 Wells (12 x 8 Well strips)
  • POTEG Lyophilized Standard: 2
  • 100X POTEG Biotinylated Detector Anitbody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • 100X Luminol Substrate: 1 x 2 mL
  • Substrate Diluent: 1 x 20 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Tissue homogenates, cell lysates and other biological fluids
• Sensitivity: 0.312 ng/mL
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: POTEG
• Gene Full Name: POTE ankyrin domain family member G
• Alias Symbols: A26C2;ACTBL1;ANKRD26-like family C member 2;cancer/testis antigen family 104, member 4;CT104.4;POTE ankyrin domain family member G;POTE14;POTE-14;POTE14alpha;POTE22;prostate, ovary, testis-expressed protein on chromosome 14;protein expressed in prostate, ovary, testis, and placenta 14.
• NCBI Gene Id: 404785
• Protein Name: POTE ankyrin domain family member G
• Description of Target: POTE ankyrin domain family member G
• Uniprot ID: Q6S5H5
• Protein Accession #: NP_001005356
• Nucleotide Accession #: NM_001005356