Catalog No: OKWB00171
Size:96T
Price: $450.00
SKU
OKWB00171
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Datasheets/ManualsPrintable datasheet for POTEC ELISA Kit (Human) (OKWB00171)
Product Info
Predicted Species ReactivityHomo sapiens|Human
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric, OD 450 nm
ELISA Kit Duration~ 3 Hours
ELISA Kit Linearity
Sample Type1:2 Dilution1:4 Dilution1:8 Dilution1:16 Dilution
Serum (n=5)91-105%86-99%86-100%89-105%
EDTA Plasma (n=5)84-100%86-101%82-95%82-97%
Heparin Plasma (n=5)87-97%84-95%83-100%81-98%
ELISA Kit PrincipleAviva Systems Biology POTEC ELISA Kit (Human) (OKWB00171) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for POTEC has been pre-coated onto a 96-well plate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for POTEC is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample POTEC captured in the well.
ELISA Kit Range31.25-2000pg/ml
ELISA Kit Recovery
Sample TypeRangeMean
Serum (n=5)85-10592
EDTA Plasma (n=5)91-10096
Heparin Plasma (n=5)87-9794
ELISA Kit ReproducibilityMean Intra-Assay Precision <10%
Mean Inter-Assay Precision <12%
ELISA Kit Component
ComponentAmount
POTEC Microplate96 Wells (12 x 8 Well strips)
POTEC Lyophilized Standard2 x 2 ng
100X POTEC Biotinylated Detector Anitbody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 20 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Cell Cultures, Tissue Homogenates, Other Biological Fluids
Sensitivity18.75 pg/mL
Assay InfoAssay Type: Quantitative Sandwich ELISA
Gene SymbolPOTEC
Gene Full NamePOTE ankyrin domain family member C
Alias SymbolsA26B2;ANKRD26-like family B member 2;cancer/testis antigen family 104, member 6;CT104.6;POTE ankyrin domain family member C;POTE18;POTE-18;prostate, ovary, testis-expressed protein on chromosome 18.
NCBI Gene Id388468
Protein NamePOTE ankyrin domain family member C
Uniprot IDB2RU33
Protein Accession #NP_001131143
Nucleotide Accession #NM_001137671
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