POTEB ELISA Kit (Human) (OKWB00162)

Catalog No: OKWB00162

Size:96T

Human POTEB ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Homo sapiens|Human
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric, OD 450 nm
• ELISA Kit Duration: ~ 3 Hours
• ELISA Kit Linearity:

  Sample Type	1:2 Dilution	1:4 Dilution	1:8 Dilution	1:16 Dilution
  Serum (n=5)	85-100%	87-103%	86-103%	89-102%
  EDTA Plasma (n=5)	83-99%	82-94%	87-96%	86-100%
  Heparin Plasma (n=5)	84-89%	81-95%	81-95%	81-96%

• ELISA Kit Principle: Aviva Systems Biology POTEB ELISA Kit (Human) (OKWB00162) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for POTEB has been pre-coated onto a 96-well plate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for POTEB is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample POTEB captured in the well.
• ELISA Kit Range: 15.625-1000pg/ml
• ELISA Kit Recovery:

  Sample Type	Range	Mean
  Serum (n=5)	86-105	97
  EDTA Plasma (n=5)	86-98	93
  Heparin Plasma (n=5)	88-105	95

• ELISA Kit Reproducibility: Mean Intra-Assay Precision <10%
  Mean Inter-Assay Precision <12%
• ELISA Kit Component:
  • POTEB Microplate: 96 Wells (12 x 8 Well strips)
  • POTEB Lyophilized Standard: 2 x 1 ng
  • 100X POTEB Biotinylated Detector Anitbody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 25X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 10 mL
  • Stop Solution: 1 x 10 mL
• Reconstitution and Storage: Store as indicated in product manual.
• Sample Type: Serum, Plasma, Cell Cultures, Tissue Homogenates, Other Biological Fluids
• Sensitivity: 9.4 pg/mL
• Assay Info: Assay Type: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: POTEB3
• Gene Full Name: POTE ankyrin domain family member B3
• Alias Symbols: ANKRD26-like family B member 1;POTE ankyrin domain family member B;POTE ankyrin domain family member B3;POTE ankyrin domain family, member B-like;POTE-15;POTEB;prostate, ovary, testis-expressed protein on chromosome 15.
• NCBI Gene Id: 102724631
• Protein Name: POTE ankyrin domain family member B3
• Uniprot ID: Q6S5H4
• Protein Accession #: NP_997238
• Nucleotide Accession #: NM_207355