POR ELISA Kit (Human) (OKEH08257)

Catalog No: OKEH08257

Size:96T

Human POR ELISA Standard Curve

Product Info

• Predicted Species Reactivity: Homo sapiens, Human
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Principle: Aviva Systems Biology POR ELISA Kit (Human) (OKEH08257) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for POR has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for POR is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample POR captured in well.
• ELISA Kit Range: 78-5000pg/mL
• ELISA Kit Recovery: Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 101% (n = 20)
• ELISA Kit Reproducibility: Mean Intra-assay CV%: <=6.0% (n = 20)
  Mean Inter-assay CV%: <=11.0% (n = 20)
• ELISA Kit Component:
  • POR Microplate: 96 Wells (12 x 8 Well strips)
  • POR Lyophilized Standard: 2
  • 100X Biotinylated POR Detector Antibody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Sample Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 25X Wash Buffer: 1 x 30 mL
  • TMB Substrate: 1 x 10 mL
  • Stop Solution: 1 x 10 mL
• Additional Information: Function: This enzyme is required for electron transfer from NADP to cytochrome P450 in microsomes. It can also provide electron transfer to heme oxygenase and cytochrome B5.
  Subcellular Location: Endoplasmic reticulum membrane Single-pass membrane protein Cytoplasmic side
  Unigene: Hs.354056
  SMR: P16435
  String: 9606.ENSP00000419970
  KEGG: hsa:5447
  MIM: 124015
  Pfam: PF00667
• Reconstitution and Storage: Store as indicated in product manual.
• Sensitivity: 38pg/mL
• Specificity: Natural and recombinant Human NADPH--cytochrome P450 reductase
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: POR
• Gene Full Name: cytochrome p450 oxidoreductase
• Alias Symbols: CPR;CYPOR;NADPH--cytochrome P450 reductase;NADPH-dependent cytochrome P450 reductase;NADPH--hemoprotein reductase;P450 (cytochrome) oxidoreductase;P450R.
• NCBI Gene Id: 5447
• Protein Name: P16435
• Description of Target: NADPH--cytochrome P450 reductase
• Uniprot ID: https://www.uniprot.org/uniprot/P16435
• Protein Accession #: NP_000932.3
• Nucleotide Accession #: NM_000941.2