Catalog No: OKCD00378 (Formerly GWB-KBBCM3)
Size:96WELLS
Price: $675.00
SKU
OKCD00378
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Datasheets/ManualsPrintable datasheet for POR ELISA Kit (Human) (OKCD00378)
Product Info
Predicted Species ReactivityHomo sapiens|Human
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric
ELISA Kit Duration3h
ELISA Kit PrincipleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cytochrome P450 Reductase (CPR). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Cytochrome P450 Reductase (CPR). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Cytochrome P450 Reductase (CPR), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Cytochrome P450 Reductase (CPR) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
ELISA Kit Range0.156-10ng/mL
ELISA Kit ReproducibilityIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Cytochrome P450 Reductase (CPR) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Cytochrome P450 Reductase (CPR) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
ELISA Kit Component
ComponentAmount
Anti-POR Microplate96 Wells (12 x 8 Well Strips)
POR Lyophilized Standard2 x 10 ng
100X Biotinylated POR Detector Antibody120 uL
Avidin/HRP Conjugate120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Reconstitution and Storage2°C to 8°C|-20°C
Sample TypeTissue homogenates, cell lysates and other biological fluids.
Sensitivity< 0.059 ng/mL
SpecificityThis assay has high sensitivity and excellent specificity for detection of Cytochrome P450 Reductase (CPR). No significant cross-reactivity or interference between Cytochrome P450 Reductase (CPR) and analogues was observed.
Assay InfoAssay Methodology: Quantitative Sandwich Immunoassay
Gene SymbolPOR
Gene Full Namecytochrome p450 oxidoreductase
Alias SymbolsCPR;CYPOR;NADPH--cytochrome P450 reductase;NADPH-dependent cytochrome P450 reductase;NADPH--hemoprotein reductase;P450 (cytochrome) oxidoreductase;P450R.
NCBI Gene Id5447
Protein NameNADPH--cytochrome P450 reductase
Description of TargetThis enzyme is required for electron transfer from NADP to cytochrome P450 in microsomes. It can also provide electron transfer to heme oxygenase and cytochrome B5.
Uniprot IDP16435
Protein Accession #NP_000932
Nucleotide Accession #NM_000941.2
Protein Size (# AA)677
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