Catalog No: OPCA04345
Price: $0.00
SKU
OPCA04345
Availability: Domestic: Antibody & Kits: 2 weeks | Proteins: 4-6 weeks | International: Antibody & Kits: 2 weeks | Proteins: 4-6 weeks
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- $55: Antibody & Protein in US
- $55 + $25/Kit in US
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Datasheets/Manuals | Printable datasheet for PONA Recombinant Protein (Slime mold) (OPCA04345) (OPCA04345) |
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Predicted Species Reactivity | Dictyostelium discoideum |
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Product Format | Liquid or Lyophilized powder |
Additional Information | Species Specificity Detail: Dictyostelium discoideum (Slime mold) |
Reconstitution and Storage | -20°C or -80°C |
Formulation | Tris-base, 50% glycerol |
Purity | Greater than 90% as determined by SDS-PAGE. |
Peptide Sequence | QYTLSVSNSASGSKCTTAVSAKLNACNTGCLNSFNIVESSNGKGLVFKTFINAACSGEYESLSQFTCAANQKIPTTSYIVSCNSTPSSNSTTDSDS |
Protein Sequence | QYTLSVSNSASGSKCTTAVSAKLNACNTGCLNSFNIVESSNGKGLVFKTFINAACSGEYESLSQFTCAANQKIPTTSYIVSCNSTPSSNSTTDSDS |
Storage Buffer | If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. |
Source | Yeast |
Protein Range | 23-118 aa |
Tag | N-terminal 6xHis-tagged |
Reference | F-actin affinity chromatography of detergent-solubilized plasma membranes: purification and initial characterization of ponticulin from Dictyostelium discoideum.Wuestehube L.J., Speicher D.W., Shariff A., Luna E.J.Methods Enzymol. 196:47-65(1991) |
Gene Symbol | ponA |
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Gene Full Name | actin binding protein |
Alias Symbols | DDB_0192061;DDB_0215380;DDB_G0293522;DDBDRAFT_0192061;DDBDRAFT_0215380. |
NCBI Gene Id | 8629357 |
Protein Name | Ponticulin |
Description of Target | Binds F-actin and nucleates actin assembly. Major high affinity link between the plasma membrane and the cortical actin network. |
Uniprot ID | P54660 |
Protein Accession # | XP_629065 |
Nucleotide Accession # | XM_629063 |
Protein Size (# AA) | Recombinant |
Molecular Weight | 11.9 kDa |
- Protocol:
- Reconstitution & Storage Instructions
- Western Blotting/Immunoblotting (WB/IB) Protocol
- Immunohistochemistry (IHC) Protocol
- Immunocytochemistry (ICC) Protocol
- Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
- Blocking Peptide Competition Protocol (BPCP)
- Immunoprecipitation (IP) Protocol
- Antibody Array (AA) Protocol
- Tips Information:
-
See our General FAQ page.
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