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Catalog No: OKCA00989
Size:96 Wells
Price: $754.00
SKU
OKCA00989
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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityRat
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~ 3.5 Hours
ELISA Kit Linearity
DilutionMean n=4 (%)Range (%)
1:2009186-95
1:40010297-107
1:8009185-97
1:16009791-103
ELISA Kit PrincipleAviva Systems Biology PON1 ELISA Kit (Rat) (OKCA00989) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. A antibody specific for PON1 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for PON1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes yellow after adding acidicstop solution. The density of yellow coloration read by absorbance at 450 nm and is quantitatively proportional to the amount of sample PON1 captured in well.
ELISA Kit Range15.6 μU/mL-1,000 μU/mL
ELISA Kit Recovery
MatrixRecovery range (%)Average(%)
Serum (n=5)9589-98
EDTA Plasma (n=4)9790-100
ELISA Kit ReproducibilityIntra-assay Precision: 3 samples with known low, middle and high level PON1 were tested with 20 replicates across one plate.
Inter-assay Precision: 3 samples with known low, middle and high level PON1 were tested on three different plates with 8 replicates, respectively.
Intra-Assay CV: <8%
Inter-Assay CV: <10%
ELISA Kit Component
ComponentAmount
PON1 Microplate96 Wells (12 x 8 Well strips)
PON1 Lyophilized Standard2
100X Biotinylated PON1 Detector Anitbody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Sample Diluent1 x 50 mL
Detector Antibody Diluent1 x 15 mL
Conjugate Diluent1 x 15 mL
25X Wash Buffer1 x 20 mL
Stop Solution1 x 10 mL
TMB Substrate1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Tissue Homogenates
Sensitivity3.9 uU/mL
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Protocol Information
Gene SymbolPON1
Gene Full NameSerum paraoxonase/arylesterase 1
Alias SymbolsPON
NCBI Gene Id100009133
Protein NameSerum paraoxonase/arylesterase 1
Description of TargetHydrolyzes the toxic metabolites of a variety of organophosphorus insecticides. Capable of hydrolyzing a broad spectrum of organophosphate substrates and lactones, and a number of aromatic carboxylic acid esters. Mediates an enzymatic protection of low density lipoproteins against oxidative modification.
Uniprot IDP27170
Protein Accession #NP_001075766.1
Nucleotide Accession #NM_001082297.1
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